• Western blot analysis of lysates from COS7 cells treated with Forskolin 40nM 30', using DARPP-32 (Phospho-Thr75) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using DARPP-32 (Phospho-Thr75) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of various cells using Phospho-DARPP-32 (T75) Polyclonal Antibody diluted at 1：500

Anti-Phospho-PPP1R1B-Thr75 antibody (41-90 aa) (STJ90243)

SKU:
STJ90243

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Protein phosphatase 1 regulatory subunit 1B-Thr75 (41-90 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPP1R1B
Gene ID: 84152
Uniprot ID: PPR1B_HUMAN
Immunogen Region: 41-90 aa
Specificity: Phospho-DARPP-32 (T75) Polyclonal Antibody detects endogenous levels of DARPP-32 protein only when phosphorylated at T75.
Immunogen: The antiserum was produced against synthesized peptide derived from the human DARPP-32 around the phosphorylation site of Thr75 at the amino acid range 41-90
Function Inhibitor of protein-phosphatase 1.
Protein Name Protein Phosphatase 1 Regulatory Subunit 1b
Darpp-32
Dopamine- And Camp-Regulated Neuronal Phosphoprotein
Database Links Reactome: R-HSA-180024
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Protein Phosphatase 1 Regulatory Subunit 1b antibody
Anti-Darpp-32 antibody
Anti-Dopamine- And Camp-Regulated Neuronal Phosphoprotein antibody
Anti-PPP1R1B antibody
Anti-DARPP32 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance