• Western blot analysis of PP1-alpha (Phospho-Thr320) Antibody. The lane on the right is blocked with the PP1-alpha (Phospho-Thr320) peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PP1-alpha (Phospho-Thr320) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PPP1CA-Thr320 antibody (281-330 aa) (STJ91134)

SKU:
STJ91134

Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Serine/threonine-protein phosphatase PP1-alpha catalytic subunit-Thr320 (281-330 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPP1CA
Gene ID: 5499
Uniprot ID: PP1A_HUMAN
Immunogen Region: 281-330 aa
Specificity: Phospho-PP1 Alpha (T320) Polyclonal Antibody detects endogenous levels of PP1 Alpha protein only when phosphorylated at T320.
Immunogen: The antiserum was produced against synthesized peptide derived from the human PP1-alpha around the phosphorylation site of Thr320 at the amino acid range 281-330
Function Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Regulates NEK2 function in terms of kinase activity and centrosome number and splitting, both in the presence and absence of radiation-induced DNA damage. Regulator of neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective. Dephosphorylates CENPA. Dephosphorylates the 'Ser-139' residue of ATG16L1 causing dissociation of ATG12-ATG5-ATG16L1 complex, thereby inhibiting autophagy. (Microbial infection) Necessary for alphaviruses replication.
Protein Name Serine/Threonine-Protein Phosphatase Pp1-Alpha Catalytic Subunit
Pp-1a
Database Links Reactome: R-HSA-163560
Reactome: R-HSA-180024
Reactome: R-HSA-2173788
Reactome: R-HSA-400253
Cellular Localisation Cytoplasm
Nucleus
Nucleoplasm
Nucleolus
Primarily Nuclear And Largely Excluded From The Nucleolus
Highly Mobile In Cells And Can Be Relocalized Through Interaction With Targeting Subunits
Nom1 Plays A Role In Targeting This Protein To The Nucleolus
In The Presence Of Ppp1r8 Relocalizes From The Nucleus To Nuclear Speckles
Shuttles Toward The Cytosol During Infection With Veev
Alternative Antibody Names Anti-Serine/Threonine-Protein Phosphatase Pp1-Alpha Catalytic Subunit antibody
Anti-Pp-1a antibody
Anti-PPP1CA antibody
Anti-PPP1A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance