Western blot analysis of various lysates using Phospho-PPIG-S376 Rabbit pAb (STJ11100983) at 1:1000 dilution. 293T cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. C2C12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-PPIG-S376 antibody (STJ11100983)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11100983
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General Information

Short DescriptionRabbit polyclonal Phospho-PPIG-S376 antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolPPIG
Gene ID9360
Uniprot IDPPIG_HUMAN
Immunogen SequenceVSSGE
SpecificityA synthetic phosphorylated peptide around S376 of human PPIG (NP_004783.2).

Additional Info

Tissue Specificity Ubiquitous.
Function PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding. May be implicated in the folding, transport, and assembly of proteins. May play an important role in the regulation of pre-mRNA splicing.
Protein Name Peptidyl-Prolyl Cis-Trans Isomerase G
Ppiase G
Peptidyl-Prolyl Isomerase G
Casp10
Clk-Associating Rs-Cyclophilin
Cars-Cyp
Cars-Cyclophilin
Sr-Cyclophilin
Sr-Cyp
Srcyp
Cyclophilin G
Rotamase G
Database Links Reactome: R-HSA-72163
Reactome: R-HSA-9692916
Cellular Localisation Nucleus Matrix
Nucleus Speckle
Colocalizes With Rna Splicing Factors At Nuclear Speckles
Alternative Antibody Names Anti-Peptidyl-Prolyl Cis-Trans Isomerase G antibody
Anti-Ppiase G antibody
Anti-Peptidyl-Prolyl Isomerase G antibody
Anti-Casp10 antibody
Anti-Clk-Associating Rs-Cyclophilin antibody
Anti-Cars-Cyp antibody
Anti-Cars-Cyclophilin antibody
Anti-Sr-Cyclophilin antibody
Anti-Sr-Cyp antibody
Anti-Srcyp antibody
Anti-Cyclophilin G antibody
Anti-Rotamase G antibody
Anti-PPIG antibody

Information sourced from Uniprot.org

Citations

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