| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Cardiac phospholamban-Ser16/T17 (1-50 aa) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000IHC-P 1:50-300 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PLN |
| Gene ID: |
5350 |
| Uniprot ID: |
PPLA_HUMAN |
| Immunogen Region: |
1-50 aa |
| Specificity: |
Phospho-PLB (S16/T17) Polyclonal Antibody detects endogenous levels of PLB protein only when phosphorylated at S16/T17. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human PLB around the phosphorylation site of Ser16 and Thr17 at the amino acid range 1-50 |
| Post Translational Modifications | Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes. Palmitoylated by ZDHHC16, promoting formation of the homopentamer. In elongated spermatids, proteolytically cleaved by SPPL2C which modulates intracellular Ca(2+) homeostasis. |
| Function | Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation. Controls intracellular Ca(2+) levels in elongated spermatids. May play a role in germ cell differentiation. |
| Protein Name | Cardiac PhospholambanPlb |
| Database Links | Reactome: R-HSA-5578775Reactome: R-HSA-936837 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Membrane ProteinSarcoplasmic Reticulum MembraneMitochondrion MembraneMembraneColocalizes With Hax1 At The Endoplasmic ReticulumColocalizes With Dmpk A The Sarcoplasmic Reticulum |
| Alternative Antibody Names | Anti-Cardiac Phospholamban antibodyAnti-Plb antibodyAnti-PLN antibodyAnti-PLB antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
