Anti-Phospho-PIK3C3-Ser249 antibody (STJ196380)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized phosho peptide around human PI3 Kinase Class III (Ser249)
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Phosphatidylinositol 3-kinase catalytic subunit type 3-Ser249 for use in WB, ELISA and IHC in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA/IHC |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Dilution Range | WB 1:500-2000IHC-P 1:50-300ELISA 2000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PIK3C3 |
| Gene ID | 5289 |
| Uniprot ID | PK3C3_HUMAN |
| Immunogen | Synthesized phosho peptide around human PI3 Kinase Class III (Ser249) |
| Specificity | This antibody detects endogenous levels of Human PI3 Kinase Class III (phospho-Ser249) |
Additional Info
| Post Translational Modifications | Ubiquitinated via 'Lys-29'- and 'Lys-48'-linked ubiquitination by UBE3C, promoting its degradation. Deubiquitination by ZRANB1/TRABID promotes its stabilization, leading to autophagosome maturation. |
| Function | Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate.different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. As part of PI3KC3-C1, promotes endoplasmic reticulum membrane curvature formation prior to vesicle budding. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes. (Microbial infection) Kinase activity is required for SARS coronavirus-2/SARS-CoV-2 replication. |
| Protein Name | Phosphatidylinositol 3-Kinase Catalytic Subunit Type 3Pi3-Kinase Type 3Pi3k Type 3Ptdins-3-Kinase Type 3Phosphatidylinositol 3-Kinase P100 SubunitPhosphoinositide-3-Kinase Class 3Hvps34 |
| Database Links | Reactome: R-HSA-109704Reactome: R-HSA-1632852Reactome: R-HSA-1660514Reactome: R-HSA-1660516Reactome: R-HSA-1660517Reactome: R-HSA-168138Reactome: R-HSA-5668599Reactome: R-HSA-9679504Reactome: R-HSA-9694676Reactome: R-HSA-9705671Reactome: R-HSA-983170 |
| Cellular Localisation | MidbodyLate EndosomeCytoplasmic VesicleAutophagosomeAs Component Of The Pi3k Complex I Localized To Pre-Autophagosome StructuresAs Component Of The Pi3k Complex Ii Localized Predominantly To EndosomesAlso Localizes To Discrete Punctae Along The Ciliary Axoneme And To The Base Of The Ciliary Axoneme |
| Alternative Antibody Names | Anti-Phosphatidylinositol 3-Kinase Catalytic Subunit Type 3 antibodyAnti-Pi3-Kinase Type 3 antibodyAnti-Pi3k Type 3 antibodyAnti-Ptdins-3-Kinase Type 3 antibodyAnti-Phosphatidylinositol 3-Kinase P100 Subunit antibodyAnti-Phosphoinositide-3-Kinase Class 3 antibodyAnti-Hvps34 antibodyAnti-PIK3C3 antibodyAnti-VPS34 antibody |
Information sourced from Uniprot.org