• Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
  • Western Blot analysis of 1 Jurkat treated with LPS, 2 Jurkat, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-PIK3C3-Ser249 antibody (STJ196380)

SKU:
STJ196380

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/ELISA/IHC
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Phosphatidylinositol 3-kinase catalytic subunit type 3-Ser249 is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
ELISA 2000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PIK3C3
Gene ID: 5289
Uniprot ID: PK3C3_HUMAN
Specificity: This antibody detects endogenous levels of Human PI3 Kinase Class III (phospho-Ser249)
Immunogen: Synthesized phosho peptide around human PI3 Kinase Class III (Ser249)
Post Translational Modifications Ubiquitinated via 'Lys-29'- and 'Lys-48'-linked ubiquitination by UBE3C, promoting its degradation. Deubiquitination by ZRANB1/TRABID promotes its stabilization, leading to autophagosome maturation.
Function Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate.different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. As part of PI3KC3-C1, promotes endoplasmic reticulum membrane curvature formation prior to vesicle budding. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes. (Microbial infection) Kinase activity is required for SARS coronavirus-2/SARS-CoV-2 replication.
Protein Name Phosphatidylinositol 3-Kinase Catalytic Subunit Type 3
Pi3-Kinase Type 3
Pi3k Type 3
Ptdins-3-Kinase Type 3
Phosphatidylinositol 3-Kinase P100 Subunit
Phosphoinositide-3-Kinase Class 3
Hvps34
Database Links Reactome: R-HSA-109704
Reactome: R-HSA-1632852
Reactome: R-HSA-1660514
Reactome: R-HSA-1660516
Reactome: R-HSA-1660517
Reactome: R-HSA-168138
Reactome: R-HSA-5668599
Reactome: R-HSA-9679504
Reactome: R-HSA-9694676
Reactome: R-HSA-9705671
Reactome: R-HSA-983170
Cellular Localisation Midbody
Late Endosome
Cytoplasmic Vesicle
Autophagosome
As Component Of The Pi3k Complex I Localized To Pre-Autophagosome Structures
As Component Of The Pi3k Complex Ii Localized Predominantly To Endosomes
Localizes Also To Discrete Punctae Along The Ciliary Axoneme And To The Base Of The Ciliary Axoneme
Alternative Antibody Names Anti-Phosphatidylinositol 3-Kinase Catalytic Subunit Type 3 antibody
Anti-Pi3-Kinase Type 3 antibody
Anti-Pi3k Type 3 antibody
Anti-Ptdins-3-Kinase Type 3 antibody
Anti-Phosphatidylinositol 3-Kinase P100 Subunit antibody
Anti-Phosphoinositide-3-Kinase Class 3 antibody
Anti-Hvps34 antibody
Anti-PIK3C3 antibody
Anti-VPS34 antibody

Information sourced from Uniprot.org

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