• Western blot analysis of extracts of HeLa cells, using Phospho-PFKFB2-S483 antibody (STJ117882) at 1:2000 dilution. HeLa cells were treated by IGF-1 (50 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of lysates from HeLa cells, using Phospho-PFKFB2-S483 Rabbit polyclonal antibody (STJ117882) at 1:2000 dilution. HeLa cells were treated by IGF-1 (50 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-Phospho-PFKFB2-S483 antibody (STJ117882)

SKU:
STJ117882

£129.50 - £642.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:5000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PFKFB2
Gene ID: 5208
Uniprot ID: F262_HUMAN
Specificity: A synthetic phosphorylated peptide around S483 of human PFKFB2 (NP_006203.2).
Immunogen Sequence: NYSVG
Tissue Specificity Heart.
Post Translational Modifications Phosphorylation by AMPK stimulates activity.
Function Synthesis and degradation of fructose 2,6-bisphosphate.
Protein Name 6-Phosphofructo-2-Kinase/Fructose-2 -6-Bisphosphatase 2
6pf-2-K/Fru-2 -6-P2ase 2
Pfk/Fbpase 2
6pf-2-K/Fru-2 -6-P2ase Heart-Type Isozyme Includes - 6-Phosphofructo-2-Kinase - Fructose-2 -6-Bisphosphatase
Database Links Reactome: R-HSA-9634600
Alternative Antibody Names Anti-6-Phosphofructo-2-Kinase/Fructose-2 -6-Bisphosphatase 2 antibody
Anti-6pf-2-K/Fru-2 -6-P2ase 2 antibody
Anti-Pfk/Fbpase 2 antibody
Anti-6pf-2-K/Fru-2 -6-P2ase Heart-Type Isozyme Includes - 6-Phosphofructo-2-Kinase - Fructose-2 -6-Bisphosphatase antibody
Anti-PFKFB2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance