• Western blot analysis of extracts of HeLa and C6 cells, using Phospho-PEA15-S104 antibody (STJ117886) at 1:2000 dilution. HeLa cells were treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. C6 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of lysates from HeLa cells, using Phospho-PEA15-S104 Rabbit polyclonal antibody (A9956). HeLa cells were treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of HeLa cells, using Phospho-PEA15-S104 polyclonal antibody (STJ117886) at 1:2000 dilution or PEA15 antibody (A9956). HeLa cells were treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of lysates from HeLa and C6 cells, using Phospho-PEA15-S104 Rabbit polyclonal antibody (STJ117886) at 1:2000 dilution. HeLa cells were treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. C6 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-PEA15-S104 antibody (STJ117886)

SKU:
STJ117886

£129.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-PEA15-S104 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PEA15
Gene ID: 8682
Uniprot ID: PEA15_HUMAN
Immunogen: A synthetic phosphorylated peptide around S104 of human PEA15 (NP_003759.1).
Immunogen Sequence: IPSAK
Tissue Specificity Ubiquitously expressed. Most abundant in tissues such as heart, brain, muscle and adipose tissue which utilize glucose as an energy source. Lower expression in glucose-producing tissues. Higher levels of expression are found in tissues from individuals with type 2 diabetes than in controls.
Post Translational Modifications Phosphorylated by protein kinase C and calcium-calmodulin-dependent protein kinase. These phosphorylation events are modulated by neurotransmitters or hormones.
Function Blocks Ras-mediated inhibition of integrin activation and modulates the ERK MAP kinase cascade. Inhibits RPS6KA3 activities by retaining it in the cytoplasm. Inhibits both TNFRSF6- and TNFRSF1A-mediated CASP8 activity and apoptosis. Regulates glucose transport by controlling both the content of SLC2A1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of SLC2A4 from the cell interior to the surface.
Protein Name Astrocytic Phosphoprotein Pea-15
15 Kda Phosphoprotein Enriched In Astrocytes
Phosphoprotein Enriched In Diabetes
Ped
Database Links Reactome: R-HSA-112409
Reactome: R-HSA-5673001
Cellular Localisation Cytoplasm
Associated With Microtubules
Alternative Antibody Names Anti-Astrocytic Phosphoprotein Pea-15 antibody
Anti-15 Kda Phosphoprotein Enriched In Astrocytes antibody
Anti-Phosphoprotein Enriched In Diabetes antibody
Anti-Ped antibody
Anti-PEA15 antibody

Information sourced from Uniprot.org

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