• Western blot analysis of extracts of various cell lines, using Phospho-PAK4/PAK5/PAK6-S474/S560/S602 rabbit monoclonal antibody (STJ11102619) at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of various cell lines, using Phospho-PAK4/PAK5/PAK6-S474/S560/S602 Rabbit monoclonal antibody (STJ11102619) at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-PAK4/PAK5/PAK6-S474/S560/S602 antibody [S9MR] (STJ11102619)

SKU:
STJ11102619

£146.50 - £642.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PAK4
PAK5
Gene ID: 10298
57144
Uniprot ID: PAK4_HUMAN
PAK5_HUMAN
Specificity: A synthetic phosphorylated peptide around S474 of human PAK4PAK4 (O96013).
Immunogen Sequence: RKSLV
Tissue Specificity O96013 - Highest expression in prostate, testis and colon.
Q9P286 - Predominantly expressed in brain.
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Pak 4 antibody
Anti-P21-Activated Kinase 4 antibody
Anti-Pak-4 antibody
Anti-PAK4 antibody
Anti-KIAA1142 antibody
Anti-Serine/Threonine-Protein Kinase Pak 5 antibody
Anti-P21-Activated Kinase 5 antibody
Anti-Pak-5 antibody
Anti-P21-Activated Kinase 7 antibody
Anti-Pak-7 antibody
Anti-PAK5 antibody
Anti-KIAA1264 antibody
Anti-PAK7 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance