Western blot analysis of various lysates using Phospho-PAK1/2/3-S144/S141/S139 Rabbit mAb (STJ11102649) at 1:1000 dilution. NIH/3T3 cells were treated by Forskolin (30 uM) at 37 °C for 30 minutes after serum-starvation overnight<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-PAK1/PAK2/PAK3-S144/S141/S139 antibody [S9MR] (STJ11102649)

SKU:
STJ11102649
$163.53 - $719.67
Free Shipping
Processing The item has been added
Enquire For Bulk Order

Covered by St John's Laboratory Guarantee

Short Description :Rabbit monoclonal Phospho-PAK1/2/3-S144/S141/S139 antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
Applications:WB/ELISA
Host:Rabbit
Reactivity:Human/Mouse/Rat
Note:STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality :Monoclonal
Clone ID :S9MR
Isotype:IgG
Conjugation:Unconjugated
Concentration:Lot specific
Purification:Affinity purification
Dilution Range:WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Formulation:PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage Instruction:Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol:PAK2
PAK1
5062
5058
5063
PAK2_HUMAN
PAK1_HUMAN
PAK3_HUMAN
Immunogen Sequence:YMSFT
Specificity:A synthetic phosphorylated peptide around S144 of human PAK1PAK1 (Q13153).
Tissue Specificity Q13177 - Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.
Q13153 - Overexpressed in gastric cancer cells and tissues (at protein level).
O75914 - Restricted to the nervous system. Highly expressed in postmitotic neurons of the developing and postnatal cerebral cortex and hippocampus.
Q13177 - Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.
Q13153 - Overexpressed in gastric cancer cells and tissues (at protein level).
O75914 - Restricted to the nervous system. Highly expressed in postmitotic neurons of the developing and postnatal cerebral cortex and hippocampus.
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Pak 2 antibody
Anti-Gamma-Pak antibody
Anti-Pak65 antibody
Anti-S6/H4 Kinase antibody
Anti-P21-Activated Kinase 2 antibody
Anti-Pak-2 antibody
Anti-P58 Cleaved Into - Pak-2p27 antibody
Anti-P27 - Pak-2p34 antibody
Anti-P34 antibody
Anti-C-T-Pak2 antibody
Anti-PAK2 antibody
Anti-Serine/Threonine-Protein Kinase Pak 1 antibody
Anti-Alpha-Pak antibody
Anti-P21-Activated Kinase 1 antibody
Anti-Pak-1 antibody
Anti-P65-Pak antibody
Anti-PAK1 antibody
Anti-Serine/Threonine-Protein Kinase Pak 3 antibody
Anti-Beta-Pak antibody
Anti-Oligophrenin-3 antibody
Anti-P21-Activated Kinase 3 antibody
Anti-Pak-3 antibody
Anti-PAK3 antibody
Anti-OPHN3 antibody
Anti-Serine/Threonine-Protein Kinase Pak 2 antibody
Anti-Gamma-Pak antibody
Anti-Pak65 antibody
Anti-S6/H4 Kinase antibody
Anti-P21-Activated Kinase 2 antibody
Anti-Pak-2 antibody
Anti-P58 Cleaved Into - Pak-2p27 antibody
Anti-P27 - Pak-2p34 antibody
Anti-P34 antibody
Anti-C-T-Pak2 antibody
Anti-PAK2 antibody
Anti-Serine/Threonine-Protein Kinase Pak 1 antibody
Anti-Alpha-Pak antibody
Anti-P21-Activated Kinase 1 antibody
Anti-Pak-1 antibody
Anti-P65-Pak antibody
Anti-PAK1 antibody
Anti-Serine/Threonine-Protein Kinase Pak 3 antibody
Anti-Beta-Pak antibody
Anti-Oligophrenin-3 antibody
Anti-P21-Activated Kinase 3 antibody
Anti-Pak-3 antibody
Anti-PAK3 antibody
Anti-OPHN3 antibody

Information sourced from Uniprot.org

Related Products

Model Name Host Clonality Reactivity Application
STJA0026807 Anti-Recombinant-Phospho-PAK1/2/3-S144 + S141 + S139 antibody [RM1B37] (STJA0026807) Rabbit Monoclonal Human/Mouse/Rat FC/IF/IHC/IP/WB
STJ11104176 Anti-PAK1/PAK2/PAK3 antibody (350-450) (STJ11104176) Rabbit Polyclonal Human/Mouse/Rat WB/ELISA
STJ11103732 Anti-Phospho-MCM2-S139 antibody (STJ11103732) Rabbit Polyclonal Human WB/ELISA
STJ117901 Anti-Phospho-PAK2-S20 antibody (STJ117901) Rabbit Polyclonal Human/Mouse/Rat WB/ELISA
STJ27513 Anti-Phospho-Histone H2A-S139 antibody (STJ27513) Rabbit Polyclonal Human/Mouse/Rat WB/IHC-P/IF/ICC/ELISA

Related Products

!