| Host: | Rabbit |
| Applications: | WB/IHC-F/IHC-P/ICC/IF/IP |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit monoclonal anti-Phospho-PAK1/2/3-Ser144/Ser141/Ser154 for use in WB, IHC-F, IHC-P, ICC, IF and IP in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Clone ID : | R04-2A4 |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Purification: | Affinity Purified |
| Concentration: | 0.3 mg/mL |
| Dilution Range: | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200IP 1:20 |
| Storage Instruction: | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
| Gene Symbol: | PAK3 |
| Gene ID: | 5063 |
| Uniprot ID: | PAK3_HUMAN |
| Immunogen: | A synthetic phosphopeptide corresponding to residues surrounding Ser144 of human PAK1 |
| Tissue Specificity | Restricted to the nervous system. Highly expressed in postmitotic neurons of the developing and postnatal cerebral cortex and hippocampus. |
| Post Translational Modifications | Autophosphorylated when activated by CDC42/p21. Neddylated. |
| Function | Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as a downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development. In hippocampal neurons, necessary for the formation of dendritic spines and excitatory synapses.this function is dependent on kinase activity and may be exerted by the regulation of actomyosin contractility through the phosphorylation of myosin II regulatory light chain (MLC). |
| Protein Name | Serine/Threonine-Protein Kinase Pak 3Beta-PakOligophrenin-3P21-Activated Kinase 3Pak-3 |
| Database Links | Reactome: R-HSA-202433Reactome: R-HSA-389359Reactome: R-HSA-3928664Reactome: R-HSA-399954Reactome: R-HSA-428540Reactome: R-HSA-5218920Reactome: R-HSA-5621575Reactome: R-HSA-5627123Reactome: R-HSA-5687128Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013409Reactome: R-HSA-9013420 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Pak 3 antibodyAnti-Beta-Pak antibodyAnti-Oligophrenin-3 antibodyAnti-P21-Activated Kinase 3 antibodyAnti-Pak-3 antibodyAnti-PAK3 antibodyAnti-OPHN3 antibody |
Information sourced from Uniprot.org
