Host: | Rabbit |
Applications: | IHC/IF/ELISA |
Reactivity: | Human/Mouse/Rat |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Rabbit polyclonal antibody anti-Phospho-BDNF/NT-3 growth factors receptor-Tyr706/Y707 (676-725 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications. |
Clonality: | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Concentration: | 1 mg/mL |
Dilution Range: | IHC 1:100-1:300 ELISA 1:10000 IF 1:50-200 |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | NTRK2 |
Gene ID: | 4915 |
Uniprot ID: | NTRK2_HUMAN |
Immunogen Region: | 676-725 aa |
Specificity: | Phospho-Trk B (Y706/Y707) Polyclonal Antibody detects endogenous levels of Trk B protein only when phosphorylated at Y706/Y707. |
Immunogen: | The antiserum was produced against synthesized peptide derived from the human Trk B around the phosphorylation site of Tyr706 and Tyr707 at the amino acid range 676-725 |
Post Translational Modifications | Phosphorylated. Undergoes ligand-mediated autophosphorylation that is required for interaction with SHC1 and PLCG1 and other downstream effectors. Isoform TrkB-T-Shc is not phosphorylated. Ubiquitinated. Undergoes polyubiquitination upon activation.regulated by NGFR. Ubiquitination regulates the internalization of the receptor. |
Function | Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia. |
Protein Name | Bdnf/Nt-3 Growth Factors Receptor Gp145-Trkb Trk-B Neurotrophic Tyrosine Kinase Receptor Type 2 Trkb Tyrosine Kinase Tropomyosin-Related Kinase B |
Database Links | Reactome: R-HSA-187024 Reactome: R-HSA-9024909 Reactome: R-HSA-9025046 Reactome: R-HSA-9026357 Reactome: R-HSA-9026519 Reactome: R-HSA-9026527 Reactome: R-HSA-9028335 Reactome: R-HSA-9028731 Reactome: R-HSA-9032500 Reactome: R-HSA-9032759 Reactome: R-HSA-9032845 |
Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Endosome Membrane Early Endosome Membrane Cell Projection Axon Dendrite Cytoplasm Perinuclear Region Postsynaptic Density Internalized To Endosomes Upon Ligand-Binding |
Alternative Antibody Names | Anti-Bdnf/Nt-3 Growth Factors Receptor antibody Anti-Gp145-Trkb antibody Anti-Trk-B antibody Anti-Neurotrophic Tyrosine Kinase Receptor Type 2 antibody Anti-Trkb Tyrosine Kinase antibody Anti-Tropomyosin-Related Kinase B antibody Anti-NTRK2 antibody Anti-TRKB antibody |
Information sourced from Uniprot.org