| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-Phospho-Nucleophosmin-Thr234 (201-250 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:10000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NPM1 |
| Gene ID: | 4869 |
| Uniprot ID: | NPM_HUMAN |
| Immunogen Region: | 201-250 aa |
| Specificity: | Phospho-B23 (T234) Polyclonal Antibody detects endogenous levels of B23 protein only when phosphorylated at T234. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human NPM around the phosphorylation site of Thr234 at the amino acid range 201-250 |
| Function | Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade. In complex with MYC enhances the transcription of MYC target genes. May act as chaperonin or cotransporter in the nucleolar localization of transcription termination factor TTF1. |
| Protein Name | Nucleophosmin Npm Nucleolar Phosphoprotein B23 Nucleolar Protein No38 Numatrin |
| Database Links | Reactome: R-HSA-180746 Reactome: R-HSA-3899300 Reactome: R-HSA-606279 Reactome: R-HSA-6804115 Reactome: R-HSA-8869496 Reactome: R-HSA-9692914 Reactome: R-HSA-9700645 Reactome: R-HSA-9725370 Reactome: R-HSA-9725371 Reactome: R-HSA-9833482 |
| Cellular Localisation | Nucleus Nucleolus Nucleoplasm Cytoplasm Cytoskeleton Microtubule Organizing Center Centrosome Generally Nucleolar But Is Translocated To The Nucleoplasm In Case Of Serum Starvation Or Treatment With Anticancer Drugs Has Been Found In The Cytoplasm In Patients With Primary Acute Myelogenous Leukemia (Aml) But Not With Secondary Aml Co-Localizes With The Methylated Form Of Rps10 In The Granular Component (Gc) Region Of The Nucleolus Colocalized With Nucleolin And Apex1 In Nucleoli Isoform 1 Of Nek2 Is Required For Its Localization To The Centrosome During Mitosis Can Shuttle Between Cytoplasm And Nucleus |
| Alternative Antibody Names | Anti-Nucleophosmin antibody Anti-Npm antibody Anti-Nucleolar Phosphoprotein B23 antibody Anti-Nucleolar Protein No38 antibody Anti-Numatrin antibody Anti-NPM1 antibody Anti-NPM antibody |
Information sourced from Uniprot.org
