• Western blot analysis of lysates from HeLa cells treated with Ca+ 40nM 30', using NFAT4 (Phospho-Ser165) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunofluorescence analysis of HeLa cells, using NFAT4 (Phospho-Ser165) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-NFATC3-Ser165 antibody (131-180 aa) (STJ90689)

SKU:
STJ90689

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Nuclear factor of activated T-cells, cytoplasmic 3-Ser165 (131-180 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NFATC3
Gene ID: 4775
Uniprot ID: NFAC3_HUMAN
Immunogen Region: 131-180 aa
Specificity: Phospho-NFATc3 (S165) Polyclonal Antibody detects endogenous levels of NFATc3 protein only when phosphorylated at S165.
Immunogen: The antiserum was produced against synthesized peptide derived from the human NFAT4 around the phosphorylation site of Ser165 at the amino acid range 131-180
Post Translational Modifications Phosphorylated by NFATC-kinase.dephosphorylated by calcineurin.
Function Acts as a regulator of transcriptional activation. Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2. Along with NFATC4, involved in embryonic heart development.
Protein Name Nuclear Factor Of Activated T-Cells - Cytoplasmic 3
Nf-Atc3
Nfatc3
Nfatx
T-Cell Transcription Factor Nfat4
Nf-At4
Nf-At4c
Database Links Reactome: R-HSA-2025928
Reactome: R-HSA-2871809
Reactome: R-HSA-5607763
Cellular Localisation Cytoplasm
Nucleus
Cytoplasmic For The Phosphorylated Form And Nuclear After Activation That Is Controlled By Calcineurin-Mediated Dephosphorylation
Rapid Nuclear Exit Of Nfatc Is Thought To Be One Mechanism By Which Cells Distinguish Between Sustained And Transient Calcium Signals
The Subcellular Localization Of Nfatc Plays A Key Role In The Regulation Of Gene Transcription
Alternative Antibody Names Anti-Nuclear Factor Of Activated T-Cells - Cytoplasmic 3 antibody
Anti-Nf-Atc3 antibody
Anti-Nfatc3 antibody
Anti-Nfatx antibody
Anti-T-Cell Transcription Factor Nfat4 antibody
Anti-Nf-At4 antibody
Anti-Nf-At4c antibody
Anti-NFATC3 antibody
Anti-NFAT4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance