• Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
  • Western Blot analysis of 1 MCF-7 treated with LPS, 2 MCF-7, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-NFATC2-Ser326 antibody (STJA0009817)

SKU:
STJA0009817

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/ELISA/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Nuclear factor of activated T-cells, cytoplasmic 2-Ser326 is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-p 1:50-300
ELISA 2000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NFATC2
Gene ID: 4773
Uniprot ID: NFAC2_HUMAN
Specificity: This antibody detects endogenous levels of Human, Mouse, Rat NFAT1 (Phospho Ser326)
Immunogen: Synthesized peptide derived from human NFAT1 (Phospho Ser326)
Tissue Specificity Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas. Isoform 1 is highly expressed in the small intestine, heart, testis, prostate, thymus, placenta and thyroid. Isoform 3 is highly expressed in stomach, uterus, placenta, trachea and thyroid.
Post Translational Modifications In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated.which is required for full transcriptional activity. Ubiquitinated in endothelial cells by RNF213 downstream of the non-canonical Wnt signaling pathway, leading to its degradation by the proteasome.
Function Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway. Is involved in the negative regulation of chondrogenesis.
Protein Name Nuclear Factor Of Activated T-Cells - Cytoplasmic 2
Nf-Atc2
Nfatc2
Nfat Pre-Existing Subunit
Nf-Atp
T-Cell Transcription Factor Nfat1
Database Links Reactome: R-HSA-2025928
Reactome: R-HSA-2871809
Reactome: R-HSA-5607763
Reactome: R-HSA-8877330
Cellular Localisation Cytoplasm
Nucleus
Cytoplasmic For The Phosphorylated Form And Nuclear After Activation That Is Controlled By Calcineurin-Mediated Dephosphorylation
Rapid Nuclear Exit Of Nfatc Is Thought To Be One Mechanism By Which Cells Distinguish Between Sustained And Transient Calcium Signals
The Subcellular Localization Of Nfatc Plays A Key Role In The Regulation Of Gene Transcription
Alternative Antibody Names Anti-Nuclear Factor Of Activated T-Cells - Cytoplasmic 2 antibody
Anti-Nf-Atc2 antibody
Anti-Nfatc2 antibody
Anti-Nfat Pre-Existing Subunit antibody
Anti-Nf-Atp antibody
Anti-T-Cell Transcription Factor Nfat1 antibody
Anti-NFATC2 antibody
Anti-NFAT1 antibody
Anti-NFATP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance