Anti-Phospho-NF2-Ser518 antibody (485-534 aa) (STJ90340)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human Merlin around the phosphorylation site of Ser518 at the amino acid range 485-534
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Merlin-Ser518 (485-534 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:5000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NF2 |
| Gene ID | 4771 |
| Uniprot ID | MERL_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human Merlin around the phosphorylation site of Ser518 at the amino acid range 485-534 |
| Immunogen Region | 485-534 aa |
| Specificity | Phospho-NF2 (S518) Polyclonal Antibody detects endogenous levels of NF2 protein only when phosphorylated at S518. |
Additional Info
| Post Translational Modifications | Phosphorylation of Ser-518 inhibits nuclear localization by disrupting the intramolecular association of the FERM domain with the C-terminal tail. The dephosphorylation of Ser-518 favors the interaction with NOP53. Ubiquitinated by the CUL4A-RBX1-DDB1-DCAF1/VprBP E3 ubiquitin-protein ligase complex for ubiquitination and subsequent proteasome-dependent degradation. |
| Function | Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex. |
| Protein Name | MerlinMoesin-Ezrin-Radixin-Like ProteinNeurofibromin-2SchwannomerlinSchwannomin |
| Database Links | Reactome: R-HSA-2029482Reactome: R-HSA-5627123 |
| Cellular Localisation | Isoform 1: Cell ProjectionFilopodium MembranePeripheral Membrane ProteinCytoplasmic SideCell ProjectionRuffle MembraneNucleusIn A Fibroblastic Cell LineIsoform 1 Is Found Homogeneously Distributed Over The Entire CellWith A Particularly Strong Staining In Ruffling Membranes And FilopodiaColocalizes With Mpp1 In Non-Myelin-Forming Schwann CellsBinds With Dcaf1 In The NucleusThe Intramolecular Association Of The Ferm Domain With The C-Terminal Tail Promotes Nuclear AccumulationThe Unphosphorylated Form Accumulates Predominantly In The Nucleus While The Phosphorylated Form Is Largely Confined To The Non-Nuclear FractionsIsoform 7: CytoplasmPerinuclear RegionCytoplasmic GranuleObserved In Cytoplasmic Granules Concentrated In A Perinuclear LocationIsoform 7 Is Absent From Ruffling Membranes And FilopodiaIsoform 9: CytoplasmIsoform 9 Is Absent From Ruffling Membranes And FilopodiaIsoform 10: NucleusCytoplasmCytoskeletonIsoform 10 Is Found Homogeneously Distributed Over The Entire Cell |
| Alternative Antibody Names | Anti-Merlin antibodyAnti-Moesin-Ezrin-Radixin-Like Protein antibodyAnti-Neurofibromin-2 antibodyAnti-Schwannomerlin antibodyAnti-Schwannomin antibodyAnti-NF2 antibodyAnti-SCH antibody |
Information sourced from Uniprot.org