Anti-Phospho-NEK9-Thr210 antibody (176-225 aa) (STJ90724)

SKU:
STJ90724
$67.62 - $360.18
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Short Description :Rabbit polyclonal anti-Phospho-Serine/threonine-protein kinase Nek9-Thr210 (176-225 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
Applications:WB/IHC/IF/ELISA
Host:Rabbit
Reactivity:Human/Mouse/Rat
Note:STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality :Polyclonal
Isotype:IgG
Conjugation:Unconjugated
Concentration:1 mg/mL
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution Range:WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Formulation:Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage Instruction:Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol:NEK9
Gene ID:91754
Uniprot ID:NEK9_HUMAN
Immunogen:The antiserum was produced against synthesized peptide derived from the human NEK9 around the phosphorylation site of Thr210 at the amino acid range 176-225
Immunogen Region:176-225 aa
Specificity:Phospho-Nek9 (T210) Polyclonal Antibody detects endogenous levels of Nek9 protein only when phosphorylated at T210.
Post Translational Modifications Autophosphorylated on serine and threonine residues. When complexed with FACT, exhibits markedly elevated phosphorylation on Thr-210. During mitosis, not phosphorylated on Thr-210. Phosphorylated by CDK1 in vitro.
Function Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively.
Protein Name Serine/Threonine-Protein Kinase Nek9
Nercc1 Kinase
Never In Mitosis A-Related Kinase 9
Nima-Related Protein Kinase 9
Nima-Related Kinase 8
Nek8
Database Links Reactome: R-HSA-2980767
Reactome: R-HSA-3301854
Reactome: R-HSA-9648025
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Nek9 antibody
Anti-Nercc1 Kinase antibody
Anti-Never In Mitosis A-Related Kinase 9 antibody
Anti-Nima-Related Protein Kinase 9 antibody
Anti-Nima-Related Kinase 8 antibody
Anti-Nek8 antibody
Anti-NEK9 antibody
Anti-KIAA1995 antibody
Anti-NEK8 antibody
Anti-NERCC antibody

Information sourced from Uniprot.org