• Immunofluorescence analysis of HeLa cells, using NEK9 (Phospho-Thr210) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from HepG2 cells, using NEK9 (Phospho-Thr210) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-NEK9-Thr210 antibody (176-225 aa) (STJ90724)

SKU:
STJ90724

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Serine/threonine-protein kinase Nek9-Thr210 (176-225 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NEK9
Gene ID: 91754
Uniprot ID: NEK9_HUMAN
Immunogen Region: 176-225 aa
Specificity: Phospho-Nek9 (T210) Polyclonal Antibody detects endogenous levels of Nek9 protein only when phosphorylated at T210.
Immunogen: The antiserum was produced against synthesized peptide derived from the human NEK9 around the phosphorylation site of Thr210 at the amino acid range 176-225
Post Translational Modifications Autophosphorylated on serine and threonine residues. When complexed with FACT, exhibits markedly elevated phosphorylation on Thr-210. During mitosis, not phosphorylated on Thr-210. Phosphorylated by CDK1 in vitro.
Function Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively.
Protein Name Serine/Threonine-Protein Kinase Nek9
Nercc1 Kinase
Never In Mitosis A-Related Kinase 9
Nima-Related Protein Kinase 9
Nima-Related Kinase 8
Nek8
Database Links Reactome: R-HSA-2980767
Reactome: R-HSA-3301854
Reactome: R-HSA-9648025
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Nek9 antibody
Anti-Nercc1 Kinase antibody
Anti-Never In Mitosis A-Related Kinase 9 antibody
Anti-Nima-Related Protein Kinase 9 antibody
Anti-Nima-Related Kinase 8 antibody
Anti-Nek8 antibody
Anti-NEK9 antibody
Anti-KIAA1995 antibody
Anti-NEK8 antibody
Anti-NERCC antibody

Information sourced from Uniprot.org

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