Anti-Phospho-MYOD1-Ser200 antibody (171-220 aa) (STJ90472)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human MYOD around the phosphorylation site of Ser200 at the amino acid range 171-220
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Myoblast determination protein 1-Ser200 (171-220 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MYOD1 |
| Gene ID | 4654 |
| Uniprot ID | MYOD1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human MYOD around the phosphorylation site of Ser200 at the amino acid range 171-220 |
| Immunogen Region | 171-220 aa |
| Specificity | Phospho-MyoD (S200) Polyclonal Antibody detects endogenous levels of MyoD protein only when phosphorylated at S200. |
Additional Info
| Function | Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins. |
| Protein Name | Myoblast Determination Protein 1Class C Basic Helix-Loop-Helix Protein 1Bhlhc1Myogenic Factor 3Myf-3 |
| Database Links | Reactome: R-HSA-525793Reactome: R-HSA-9839394 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Myoblast Determination Protein 1 antibodyAnti-Class C Basic Helix-Loop-Helix Protein 1 antibodyAnti-Bhlhc1 antibodyAnti-Myogenic Factor 3 antibodyAnti-Myf-3 antibodyAnti-MYOD1 antibodyAnti-BHLHC1 antibodyAnti-MYF3 antibodyAnti-MYOD antibody |
Information sourced from Uniprot.org