• Western blot analysis of extracts of various cell lines, using Phospho-MYL9-T18/S19 antibody (STJ11101086) at 1:1000 dilution. 293T cells and C2C12 and C6 cells were treated by ATP (5 mM) at 30 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of MYL9 in paraffin-embedded Human gastric cancer using Phospho-MYL9-T18/S19 antibody (STJ11101086) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded Rat ovary using Phospho-MYL9-T18/S19 antibody (STJ11101086) at dilution of 1:100 (20x lens).
  • Immunohistochemistry analysis of MYL9 in paraffin-embedded Rat ovary using Phospho-MYL9-T18/S19 antibody (STJ11101086) at dilution of 1:100 (20x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded Human gastric cancer using Phospho-MYL9-T18/S19 antibody (STJ11101086) at dilution of 1:100 (40x lens).
  • Western blot analysis of HeLa, using Phospho-MYL9-T18/S19 antibody (STJ11101086) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-MYL9-T18/S19 antibody (STJ11101086)

SKU:
STJ11101086

£129.50 - £626.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-MYL9-T18/S19 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYL9
Gene ID: 10398
Uniprot ID: MYL9_HUMAN
Immunogen: A synthetic phosphorylated peptide around T18 & S19 of human MYL9 (NP_006088.2).
Immunogen Sequence: PQRATSNVFA
Tissue Specificity Smooth muscle tissues and in some, but not all, nonmuscle cells.
Post Translational Modifications Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. Phosphorylation is required for myotube formation.
Function Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. In myoblasts, may regulate PIEZO1-dependent cortical actomyosin assembly involved in myotube formation.
Protein Name Myosin Regulatory Light Polypeptide 9
20 Kda Myosin Light Chain
Lc20
Mlc-2c
Myosin Rlc
Myosin Regulatory Light Chain 2 - Smooth Muscle Isoform
Myosin Regulatory Light Chain 9
Myosin Regulatory Light Chain Mrlc1
Database Links Reactome: R-HSA-3928663
Reactome: R-HSA-416572
Reactome: R-HSA-445355
Reactome: R-HSA-5625740
Reactome: R-HSA-5625900
Reactome: R-HSA-5627117
Reactome: R-HSA-5627123
Reactome: R-HSA-8936459
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Cortex
Colocalizes With F-Actin
Myh9 And Piezo1 At The Actomyosin Cortex In Myoblasts
Alternative Antibody Names Anti-Myosin Regulatory Light Polypeptide 9 antibody
Anti-20 Kda Myosin Light Chain antibody
Anti-Lc20 antibody
Anti-Mlc-2c antibody
Anti-Myosin Rlc antibody
Anti-Myosin Regulatory Light Chain 2 - Smooth Muscle Isoform antibody
Anti-Myosin Regulatory Light Chain 9 antibody
Anti-Myosin Regulatory Light Chain Mrlc1 antibody
Anti-MYL9 antibody
Anti-MLC2 antibody
Anti-MRLC1 antibody
Anti-MYRL2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance