• Western blot analysis of extracts of 293T cells, using Phospho-MYH9-S1943 antibody (STJ117900) at 1:2000 dilution. 293T cell lysates were treated by CIP at 37°C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 30s.
  • Western blot analysis of lysates from 293T cells, using Phospho-MYH9-S1943 Rabbit polyclonal antibody (STJ117900) at 1:2000 dilution. 293T cell lysates were treated by CIP at 37°C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-Phospho-MYH9-S1943 antibody (STJ117900)

SKU:
STJ117900

£129.50 - £639.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-MYH9-S1943 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYH9
Gene ID: 4627
Uniprot ID: MYH9_HUMAN
Immunogen: A synthetic phosphorylated peptide around S1943 of human MYH9 (NP_002464.1).
Immunogen Sequence: DGSDE
Tissue Specificity In the kidney, expressed in the glomeruli. Also expressed in leukocytes.
Post Translational Modifications ISGylated. Ubiquitination.
Function Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Required for cortical actin clearance prior to oocyte exocytosis. Promotes cell motility in conjunction with S100A4. During cell spreading, plays an important role in cytoskeleton reorganization, focal contact formation (in the margins but not the central part of spreading cells), and lamellipodial retraction.this function is mechanically antagonized by MYH10.
Protein Name Myosin-9
Cellular Myosin Heavy Chain - Type A
Myosin Heavy Chain 9
Myosin Heavy Chain - Non-Muscle Iia
Non-Muscle Myosin Heavy Chain A
Nmmhc-A
Non-Muscle Myosin Heavy Chain Iia
Nmmhc Ii-A
Nmmhc-Iia
Database Links Reactome: R-HSA-1445148
Reactome: R-HSA-2029482
Reactome: R-HSA-3928663
Reactome: R-HSA-416572
Reactome: R-HSA-5625740
Reactome: R-HSA-5625900
Reactome: R-HSA-5627117
Reactome: R-HSA-5627123
Reactome: R-HSA-9662360
Reactome: R-HSA-9662361
Reactome: R-HSA-9662834
Reactome: R-HSA-9664422
Reactome: R-HSA-9725370
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Cortex
Cytoplasmic Vesicle
Secretory Vesicle
Cortical Granule
Colocalizes With Actin Filaments At Lamellipodia Margins And At The Leading Edge Of Migrating Cells
In Retinal Pigment Epithelial Cells
Predominantly Localized To Stress Fiber-Like Structures With Some Localization To Cytoplasmic Puncta
Alternative Antibody Names Anti-Myosin-9 antibody
Anti-Cellular Myosin Heavy Chain - Type A antibody
Anti-Myosin Heavy Chain 9 antibody
Anti-Myosin Heavy Chain - Non-Muscle Iia antibody
Anti-Non-Muscle Myosin Heavy Chain A antibody
Anti-Nmmhc-A antibody
Anti-Non-Muscle Myosin Heavy Chain Iia antibody
Anti-Nmmhc Ii-A antibody
Anti-Nmmhc-Iia antibody
Anti-MYH9 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance