Anti-Phospho-MLK1/2-Thr312/266 antibody (281-330 aa) (STJ91210)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human MLK1/2 around the phosphorylation site of Thr312/266 at the amino acid range 281-330
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Mitogen-activated protein kinase kinase kinase 9 and Mitogen-activated protein kinase kinase kinase 10-Thr312/266 (281-330 aa) for use in IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution re |
| Applications | IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MAP3K9MAP3K10 |
| Gene ID | 42934294 |
| Uniprot ID | M3K9_HUMANM3K10_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human MLK1/2 around the phosphorylation site of Thr312/266 at the amino acid range 281-330 |
| Immunogen Region | 281-330 aa |
| Specificity | Phospho-MLK1/2 (T312/266) Polyclonal Antibody detects endogenous levels of MLK1/2 protein only when phosphorylated at T312/266. |
Additional Info
| Post Translational Modifications | Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site. Autophosphorylation also occurs on Thr-304 and Ser-308. |
| Function | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade through the phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7 which in turn activate the JNKs. The MKK/JNK signaling pathway regulates stress response via activator protein-1 (JUN) and GATA4 transcription factors. Plays also a role in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. |
| Protein Name | Mitogen-Activated Protein Kinase Kinase Kinase 9Mixed Lineage Kinase 1 |
| Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase Kinase Kinase 9 antibodyAnti-Mixed Lineage Kinase 1 antibodyAnti-MAP3K9 antibodyAnti-MLK1 antibodyAnti-PRKE1 antibody |
Information sourced from Uniprot.org