Anti-Phospho-MKNK1-Thr385 antibody (351-400 aa) (STJ90984)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human Mnk1 around the phosphorylation site of Thr385 at the amino acid range 351-400
General Information
| Short Description | Rabbit polyclonal anti-Phospho-MAP kinase-interacting serine/threonine-protein kinase 1-Thr385 (351-400 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:20000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MKNK1 |
| Gene ID | 8569 |
| Uniprot ID | MKNK1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human Mnk1 around the phosphorylation site of Thr385 at the amino acid range 351-400 |
| Immunogen Region | 351-400 aa |
| Specificity | Phospho-Mnk1 (T385) Polyclonal Antibody detects endogenous levels of Mnk1 protein only when phosphorylated at T385. |
Additional Info
| Post Translational Modifications | Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase. MAPK3/ERK1 is one of the kinases which activate MKNK1/MNK1. Phosphorylation by PAK2 leads to a reduced phosphorylation of EIF4G1. |
| Function | May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap. |
| Protein Name | Map Kinase-Interacting Serine/Threonine-Protein Kinase 1Map Kinase Signal-Integrating Kinase 1Mapk Signal-Integrating Kinase 1Mnk1 |
| Database Links | Reactome: R-HSA-1295596 |
| Cellular Localisation | Isoform 2: CytoplasmIsoform 3: CytoplasmNucleus |
| Alternative Antibody Names | Anti-Map Kinase-Interacting Serine/Threonine-Protein Kinase 1 antibodyAnti-Map Kinase Signal-Integrating Kinase 1 antibodyAnti-Mapk Signal-Integrating Kinase 1 antibodyAnti-Mnk1 antibodyAnti-MKNK1 antibodyAnti-MNK1 antibody |
Information sourced from Uniprot.org