• Western blot analysis of lysates from COLO205 cells treated with PMA 125ng/ml 30', using Mnk1 (Phospho-Thr385) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human brain, using Mnk1 (Phospho-Thr385) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MKNK1-Thr385 antibody (351-400 aa) (STJ90984)

SKU:
STJ90984

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-MAP kinase-interacting serine/threonine-protein kinase 1-Thr385 (351-400 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MKNK1
Gene ID: 8569
Uniprot ID: MKNK1_HUMAN
Immunogen Region: 351-400 aa
Specificity: Phospho-Mnk1 (T385) Polyclonal Antibody detects endogenous levels of Mnk1 protein only when phosphorylated at T385.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Mnk1 around the phosphorylation site of Thr385 at the amino acid range 351-400
Post Translational Modifications Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase. MAPK3/ERK1 is one of the kinases which activate MKNK1/MNK1. Phosphorylation by PAK2 leads to a reduced phosphorylation of EIF4G1.
Function May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
Protein Name Map Kinase-Interacting Serine/Threonine-Protein Kinase 1
Map Kinase Signal-Integrating Kinase 1
Mapk Signal-Integrating Kinase 1
Mnk1
Database Links Reactome: R-HSA-1295596
Cellular Localisation Isoform 2: Cytoplasm
Isoform 3: Cytoplasm
Nucleus
Alternative Antibody Names Anti-Map Kinase-Interacting Serine/Threonine-Protein Kinase 1 antibody
Anti-Map Kinase Signal-Integrating Kinase 1 antibody
Anti-Mapk Signal-Integrating Kinase 1 antibody
Anti-Mnk1 antibody
Anti-MKNK1 antibody
Anti-MNK1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance