| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Monkey |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-Mediator of RNA polymerase II transcription subunit 1-Thr1457 (1423-1472 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:5000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MED1 |
| Gene ID: | 5469 |
| Uniprot ID: | MED1_HUMAN |
| Immunogen Region: | 1423-1472 aa |
| Specificity: | Phospho-TRAP220 (T1457) Polyclonal Antibody detects endogenous levels of TRAP220 protein only when phosphorylated at T1457. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human PPAR-BP around the phosphorylation site of Thr1457 at the amino acid range 1423-1472 |
| Post Translational Modifications | Phosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. Phosphorylation increases its interaction with PSIP1. |
| Function | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells. |
| Protein Name | Mediator Of Rna Polymerase Ii Transcription Subunit 1Activator-Recruited Cofactor 205 Kda ComponentArc205Mediator Complex Subunit 1Peroxisome Proliferator-Activated Receptor-Binding ProteinPbpPpar-Binding ProteinThyroid Hormone Receptor-Associated Protein Complex 220 Kda ComponentTrap220Thyroid Receptor-Interacting Protein 2Tr-Interacting Protein 2Trip-2Vitamin D Receptor-Interacting Protein Complex Component Drip205P53 Regulatory Protein Rb18a |
| Database Links | Reactome: R-HSA-1368082Reactome: R-HSA-1368108Reactome: R-HSA-1989781Reactome: R-HSA-212436Reactome: R-HSA-2151201Reactome: R-HSA-2426168Reactome: R-HSA-381340Reactome: R-HSA-383280Reactome: R-HSA-400206Reactome: R-HSA-400253Reactome: R-HSA-9018519Reactome: R-HSA-9707564Reactome: R-HSA-9707616Reactome: R-HSA-9833110 |
| Cellular Localisation | NucleusA Subset Of The Protein May Enter The Nucleolus Subsequent To Phosphorylation By Mapk1 Or Mapk3 |
| Alternative Antibody Names | Anti-Mediator Of Rna Polymerase Ii Transcription Subunit 1 antibodyAnti-Activator-Recruited Cofactor 205 Kda Component antibodyAnti-Arc205 antibodyAnti-Mediator Complex Subunit 1 antibodyAnti-Peroxisome Proliferator-Activated Receptor-Binding Protein antibodyAnti-Pbp antibodyAnti-Ppar-Binding Protein antibodyAnti-Thyroid Hormone Receptor-Associated Protein Complex 220 Kda Component antibodyAnti-Trap220 antibodyAnti-Thyroid Receptor-Interacting Protein 2 antibodyAnti-Tr-Interacting Protein 2 antibodyAnti-Trip-2 antibodyAnti-Vitamin D Receptor-Interacting Protein Complex Component Drip205 antibodyAnti-P53 Regulatory Protein Rb18a antibodyAnti-MED1 antibodyAnti-ARC205 antibodyAnti-CRSP1 antibodyAnti-CRSP200 antibodyAnti-DRIP205 antibodyAnti-DRIP230 antibodyAnti-PBP antibodyAnti-PPARBP antibodyAnti-PPARGBP antibodyAnti-RB18A antibodyAnti-TRAP220 antibodyAnti-TRIP2 antibody |
Information sourced from Uniprot.org
