• Western blot of mouse whole brain lysate showing specific immunolabeling of the ~55 kDa truncated MeCP2 protein phosphorylated at Ser421 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-MECP2-Ser421 antibody (STJA0003681)

SKU:
STJA0003681-100

£442.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat/Human/Non-Human Primates
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-MeCP2-Ser421 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: This antibody was antigen affinity purified from pooled serum.
Dilution Range: WB 1:1000
IHC
ICC
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Mecp2
Gene ID: 17257
Uniprot ID: MECP2_MOUSE
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser421 conjugated to KLH.
Post Translational Modifications Phosphorylated on Ser-421 by CaMK2 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation. Does not seem to be phosphorylated on Ser-421 in other tissues.
Function Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).
Protein Name Methyl-Cpg-Binding Protein 2
Mecp-2 Protein
Mecp2
Database Links Reactome: R-MMU-9022692 Q9Z2D6-1
Cellular Localisation Nucleus
Colocalized With Methyl-Cpg In The Genome
Colocalized With Tbl1x To The Heterochromatin Foci
Alternative Antibody Names Anti-Methyl-Cpg-Binding Protein 2 antibody
Anti-Mecp-2 Protein antibody
Anti-Mecp2 antibody
Anti-Mecp2 antibody

Information sourced from Uniprot.org

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