| Host: |
Rabbit |
| Applications: |
IHC/IF/ELISA |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Mediator of DNA damage checkpoint protein 1-Ser513 (479-528 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MDC1 |
| Gene ID: |
9656 |
| Uniprot ID: |
MDC1_HUMAN |
| Immunogen Region: |
479-528 aa |
| Specificity: |
Phospho-MDC1 (S513) Polyclonal Antibody detects endogenous levels of MDC1 protein only when phosphorylated at S513. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human MDC1 around the phosphorylation site of Ser513 at the amino acid range 479-528 |
| Post Translational Modifications | Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint. Phosphorylation at Thr-4 by ATM stabilizes and enhances homodimerization via the FHA domain. Sumoylation at Lys-1840 by PIAS4 following DNA damage promotes ubiquitin-mediated degradation. Ubiquitinated by RNF4, leading to proteasomal degradation.undergoes 'Lys-48'-linked polyubiquitination. |
| Function | Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1. |
| Protein Name | Mediator Of Dna Damage Checkpoint Protein 1Nuclear Factor With Brct Domains 1 |
| Database Links | Reactome: R-HSA-3108214Reactome: R-HSA-5693565Reactome: R-HSA-5693571Reactome: R-HSA-5693607Reactome: R-HSA-6796648Reactome: R-HSA-69473 |
| Cellular Localisation | NucleusChromosomeAssociated With ChromatinRelocalizes To Discrete Nuclear Foci Following Dna DamageThis Requires 'Ser-139' Phosphorylation Of H2axColocalizes With Aptx At Sites Of Dna Double-Strand Breaks |
| Alternative Antibody Names | Anti-Mediator Of Dna Damage Checkpoint Protein 1 antibodyAnti-Nuclear Factor With Brct Domains 1 antibodyAnti-MDC1 antibodyAnti-KIAA0170 antibodyAnti-NFBD1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
