• Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MARK1/2/3/4 (Phospho-Thr215) Antibody
  • Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-MARK1/2/3/4-Thr215 antibody (181-230 aa) (STJ91168)

SKU:
STJ91168

$62.10 - $305.10
Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Serine/threonine-protein kinase MARK1 and Serine/threonine-protein kinase MARK2 and MAP/microtubule affinity-regulating kinase 3 and MAP/microtubule affinity-regulating kinase 4-Thr215 (181-230 aa) is suitable
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MARK4
MARK1
57787
4139
2011
MARK4_HUMAN
MARK1_HUMAN
MARK2_HUMAN
Immunogen Region: 181-230 aa
Specificity: Phospho-MARK1/2/3/4 (T215) Polyclonal Antibody detects endogenous levels of MARK1/2/3/4 protein only when phosphorylated at T215.
Immunogen: The antiserum was produced against synthesized peptide derived from the human MARK1/2/3/4 around the phosphorylation site of Thr215 at the amino acid range 181-230
Post Translational Modifications Phosphorylation at Thr-613 by PRKCZ/aPKC in polarized epithelial cells inhibits the kinase activity. Phosphorylated at Thr-215 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylation at Thr-215 by TAOK1 activates the kinase activity, leading to phosphorylation and detachment of MAPT/TAU from microtubules. Phosphorylation at Ser-219 by GSK3-beta (GSK3B) inhibits the kinase activity.
Function Serine/threonine-protein kinase. Involved in cell polarity and microtubule dynamics regulation. Phosphorylates DCX, MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU. Involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3).
Protein Name Serine/Threonine-Protein Kinase Mark1
Map/Microtubule Affinity-Regulating Kinase 1
Par1 Homolog C
Par-1c
Par1c
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasm
Cytoskeleton
Cell Projection
Dendrite
Appears To Localize To An Intracellular Network
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Mark1 antibody
Anti-Map/Microtubule Affinity-Regulating Kinase 1 antibody
Anti-Par1 Homolog C antibody
Anti-Par-1c antibody
Anti-Par1c antibody
Anti-MARK1 antibody
Anti-KIAA1477 antibody
Anti-MARK antibody

Information sourced from Uniprot.org

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