Anti-Phospho-MARCKS-Ser163 antibody (136-185 aa) (STJ91287)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human MARCKS around the phosphorylation site of Ser163 at the amino acid range 136-185
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Myristoylated alanine-rich C-kinase substrate-Ser163 (136-185 aa) for use in IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MARCKS |
| Gene ID | 4082 |
| Uniprot ID | MARCS_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human MARCKS around the phosphorylation site of Ser163 at the amino acid range 136-185 |
| Immunogen Region | 136-185 aa |
| Specificity | Phospho-MARCKS (S163) Polyclonal Antibody detects endogenous levels of MARCKS protein only when phosphorylated at S163. |
Additional Info
| Post Translational Modifications | Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity. PKC-mediated phosphorylation increases 4 to 5-fold upon TNF-alpha or LPS induction. Myristoylated. A proper myristoylation is essential for the proper distribution to the plasma membrane. Acetylated at Lys-172 by KAT5.acetylation is required for its subsequent phosphorylation. Deacetylated by SIRT2. |
| Function | Membrane-associated protein that plays a role in the structural modulation of the actin cytoskeleton, chemotaxis, motility, cell adhesion, phagocytosis, and exocytosis through lipid sequestering and/or protein docking to membranes. Thus, exerts an influence on a plethora of physiological processes, such as embryonic development, tissue regeneration, neuronal plasticity, and inflammation. Sequesters phosphatidylinositol 4,5-bisphosphate (PIP2) at lipid rafts in the plasma membrane of quiescent cells, an action reversed by protein kinase C, ultimately inhibiting exocytosis. During inflammation, promotes the migration and adhesion of inflammatory cells and the secretion of cytokines such as tumor necrosis factor (TNF), particularly in macrophages. Plays an essential role in bacteria-induced intracellular reactive oxygen species (ROS) formation in the monocytic cell type. Participates in the regulation of neurite initiation and outgrowth by interacting with components of cellular machinery including CDC42 that regulates cell shape and process extension through modulation of the cytoskeleton. Plays also a role in axon development by mediating docking and fusion of RAB10-positive vesicles with the plasma membrane. |
| Protein Name | Myristoylated Alanine-Rich C-Kinase SubstrateMarcksProtein Kinase C Substrate - 80 Kda Protein - Light Chain80k-L ProteinPkcsl |
| Database Links | Reactome: R-HSA-399997 |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmCytoskeletonPkc-Dependent Phosphorylation Displaces Marcks From The Cell Membrane And Subsequent Dephosphorylation Is Accompanied By Its Reassociation With The Membrane |
| Alternative Antibody Names | Anti-Myristoylated Alanine-Rich C-Kinase Substrate antibodyAnti-Marcks antibodyAnti-Protein Kinase C Substrate - 80 Kda Protein - Light Chain antibodyAnti-80k-L Protein antibodyAnti-Pkcsl antibodyAnti-MARCKS antibodyAnti-MACS antibodyAnti-PRKCSL antibody |
Information sourced from Uniprot.org