• Western blot analysis of extracts of various cell lines, using Phospho-ERK1-T202 antibody (STJ22261) at 1:1000 dilution. Jurkat and NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Jurkat cells were treated by ATP (5 mM) at 30 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of methanol-fixed HeLa cells showing cytoplasmic, nuclear staining using Phospho-ERK1-T202 antibody (STJ22261).
  • Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Phospho-ERK1-T202 antibody (STJ22261).
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Phospho-ERK1-T202 antibody (STJ22261). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of methanol-fixed HeLa cells showing cytoplasmic, nuclear staining using Phospho-ERK1-T202 antibody (STJ22261).
  • Western blot analysis of extracts of various cell lines, using Phospho-ERK1-T202 antibody (STJ22261) at 1:1000 dilution. Jurkat and NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Jurkat cells were treated by ATP (5 mM) at 30 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-MAPK3-T202 antibody (STJ22261)

SKU:
STJ22261

£129.50 - £642.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:100
IF/ICC:1:20-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAPK3
MAPK1
Gene ID: 5595
5594
Uniprot ID: MK03_HUMAN
MK01_HUMAN
Specificity: A synthetic phosphorylated peptide around T202 of human ERK1/ERK2 (NP_002737.2).
Immunogen Sequence: FLTEY
Tissue Specificity
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase 3 antibody
Anti-Map Kinase 3 antibody
Anti-Mapk 3 antibody
Anti-Ert2 antibody
Anti-Extracellular Signal-Regulated Kinase 1 antibody
Anti-Erk-1 antibody
Anti-Insulin-Stimulated Map2 Kinase antibody
Anti-Map Kinase Isoform P44 antibody
Anti-P44-Mapk antibody
Anti-Microtubule-Associated Protein 2 Kinase antibody
Anti-P44-Erk1 antibody
Anti-MAPK3 antibody
Anti-ERK1 antibody
Anti-PRKM3 antibody
Anti-Mitogen-Activated Protein Kinase 1 antibody
Anti-Map Kinase 1 antibody
Anti-Mapk 1 antibody
Anti-Ert1 antibody
Anti-Extracellular Signal-Regulated Kinase 2 antibody
Anti-Erk-2 antibody
Anti-Map Kinase Isoform P42 antibody
Anti-P42-Mapk antibody
Anti-Mitogen-Activated Protein Kinase 2 antibody
Anti-Map Kinase 2 antibody
Anti-Mapk 2 antibody
Anti-MAPK1 antibody
Anti-ERK2 antibody
Anti-PRKM1 antibody
Anti-PRKM2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance