• Western blot analysis of lysates from Jurkat cells, using ERK8 (Phospho-Thr175+Tyr177) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of Jurkat cells using Phospho-ERK 8 (T175/Y177) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-MAPK15-Thr175/Y177 antibody (141-190 aa) (STJ90517)

SKU:
STJ90517

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Mitogen-activated protein kinase 15-Thr175/Y177 (141-190 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAPK15
Gene ID: 225689
Uniprot ID: MK15_HUMAN
Immunogen Region: 141-190 aa
Specificity: Phospho-ERK 8 (T175/Y177) Polyclonal Antibody detects endogenous levels of ERK 8 protein only when phosphorylated at T175/Y177.
Immunogen: The antiserum was produced against synthesized peptide derived from the human ERK8 around the phosphorylation site of Thr175 and Tyr177 at the amino acid range 141-190
Post Translational Modifications Autophosphorylated on Thr-175 and Tyr-177.activates the enzyme. Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase.
Function Atypical MAPK protein that regulates several process such as autophagy, ciliogenesis, protein trafficking/secretion and genome integrity, in a kinase activity-dependent manner. Controls both, basal and starvation-induced autophagy throught its interaction with GABARAP, MAP1LC3B and GABARAPL1 leading to autophagosome formation, SQSTM1 degradation and reduced MAP1LC3B inhibitory phosphorylation. Regulates primary cilium formation and the localization of ciliary proteins involved in cilium structure, transport, and signaling. Prevents the relocation of the sugar-adding enzymes from the Golgi to the endoplasmic reticulum, thereby restricting the production of sugar-coated proteins. Upon amino-acid starvation, mediates transitional endoplasmic reticulum site disassembly and inhibition of secretion. Binds to chromatin leading to MAPK15 activation and interaction with PCNA, that which protects genomic integrity by inhibiting MDM2-mediated degradation of PCNA. Regulates DA transporter (DAT) activity and protein expression via activation of RhoA. In response to H(2)O(2) treatment phosphorylates ELAVL1, thus preventing it from binding to the PDCD4 3'UTR and rendering the PDCD4 mRNA accessible to miR-21 and leading to its degradation and loss of protein expression. Also functions in a kinase activity-independent manner as a negative regulator of growth. Phosphorylates in vitro FOS and MBP. During oocyte maturation, plays a key role in the microtubule organization and meiotic cell cycle progression in oocytes, fertilized eggs, and early embryos. Interacts with ESRRA promoting its re-localization from the nucleus to the cytoplasm and then prevents its transcriptional activity.
Protein Name Mitogen-Activated Protein Kinase 15
Map Kinase 15
Mapk 15
Extracellular Signal-Regulated Kinase 7
Erk-7
Extracellular Signal-Regulated Kinase 8
Erk-8
Cellular Localisation Cytoplasm
Cytoskeleton
Cilium Basal Body
Cell Junction
Tight Junction
Microtubule Organizing Center
Centrosome
Centriole
Cytoplasmic Vesicle
Autophagosome
Golgi Apparatus
Nucleus
Spindle
Co-Localizes To The Cytoplasm Only In Presence Of Esrra
Translocates To The Nucleus Upon Activation
At Prometaphase I
Metaphase I (Mi)
Anaphase I
Telophase I
And Metaphase Ii (Mii) Stages
Is Stably Detected At The Spindle
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase 15 antibody
Anti-Map Kinase 15 antibody
Anti-Mapk 15 antibody
Anti-Extracellular Signal-Regulated Kinase 7 antibody
Anti-Erk-7 antibody
Anti-Extracellular Signal-Regulated Kinase 8 antibody
Anti-Erk-8 antibody
Anti-MAPK15 antibody
Anti-ERK7 antibody
Anti-ERK8 antibody

Information sourced from Uniprot.org

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