Anti-Phospho-MAP2K7-Ser271 antibody (236-285 aa) (STJ90950)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human MAP2K7 around the phosphorylation site of Ser271 at the amino acid range 236-285
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Dual specificity mitogen-activated protein kinase kinase 7-Ser271 (236-285 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:20000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MAP2K7 |
| Gene ID | 5609 |
| Uniprot ID | MP2K7_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human MAP2K7 around the phosphorylation site of Ser271 at the amino acid range 236-285 |
| Immunogen Region | 236-285 aa |
| Specificity | Phospho-MEK-7 (S271) Polyclonal Antibody detects endogenous levels of MEK-7 protein only when phosphorylated at S271. |
Additional Info
| Post Translational Modifications | Activated by phosphorylation on Ser-271 and Thr-275 by MAP kinase kinase kinases (MAP3Ks). |
| Function | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by pro-inflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Part of a non-canonical MAPK signaling pathway, composed of the upstream MAP3K12 kinase and downstream MAP kinases MAPK1/ERK2 and MAPK3/ERK1, that enhances the AP-1-mediated transcription of APP in response to APOE. |
| Protein Name | Dual Specificity Mitogen-Activated Protein Kinase Kinase 7Map Kinase Kinase 7Mapkk 7Jnk-Activating Kinase 2Mapk/Erk Kinase 7Mek 7Stress-Activated Protein Kinase Kinase 4Sapk Kinase 4Sapkk-4Sapkk4C-Jun N-Terminal Kinase Kinase 2Jnk Kinase 2Jnkk 2 |
| Database Links | Reactome: R-HSA-2559580Reactome: R-HSA-2871796Reactome: R-HSA-450321Reactome: R-HSA-5210891 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 7 antibodyAnti-Map Kinase Kinase 7 antibodyAnti-Mapkk 7 antibodyAnti-Jnk-Activating Kinase 2 antibodyAnti-Mapk/Erk Kinase 7 antibodyAnti-Mek 7 antibodyAnti-Stress-Activated Protein Kinase Kinase 4 antibodyAnti-Sapk Kinase 4 antibodyAnti-Sapkk-4 antibodyAnti-Sapkk4 antibodyAnti-C-Jun N-Terminal Kinase Kinase 2 antibodyAnti-Jnk Kinase 2 antibodyAnti-Jnkk 2 antibodyAnti-MAP2K7 antibodyAnti-JNKK2 antibodyAnti-MEK7 antibodyAnti-MKK7 antibodyAnti-PRKMK7 antibodyAnti-SKK4 antibody |
Information sourced from Uniprot.org