| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Dual specificity mitogen-activated protein kinase kinase 7-Ser271 (236-285 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000IHC 1:100-1:300ELISA 1:20000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MAP2K7 |
| Gene ID: |
5609 |
| Uniprot ID: |
MP2K7_HUMAN |
| Immunogen Region: |
236-285 aa |
| Specificity: |
Phospho-MEK-7 (S271) Polyclonal Antibody detects endogenous levels of MEK-7 protein only when phosphorylated at S271. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human MAP2K7 around the phosphorylation site of Ser271 at the amino acid range 236-285 |
| Post Translational Modifications | Activated by phosphorylation on Ser-271 and Thr-275 by MAP kinase kinase kinases (MAP3Ks). |
| Function | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by pro-inflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Part of a non-canonical MAPK signaling pathway, composed of the upstream MAP3K12 kinase and downstream MAP kinases MAPK1/ERK2 and MAPK3/ERK1, that enhances the AP-1-mediated transcription of APP in response to APOE. |
| Protein Name | Dual Specificity Mitogen-Activated Protein Kinase Kinase 7Map Kinase Kinase 7Mapkk 7Jnk-Activating Kinase 2Mapk/Erk Kinase 7Mek 7Stress-Activated Protein Kinase Kinase 4Sapk Kinase 4Sapkk-4Sapkk4C-Jun N-Terminal Kinase Kinase 2Jnk Kinase 2Jnkk 2 |
| Database Links | Reactome: R-HSA-2559580Reactome: R-HSA-2871796Reactome: R-HSA-450321Reactome: R-HSA-5210891 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 7 antibodyAnti-Map Kinase Kinase 7 antibodyAnti-Mapkk 7 antibodyAnti-Jnk-Activating Kinase 2 antibodyAnti-Mapk/Erk Kinase 7 antibodyAnti-Mek 7 antibodyAnti-Stress-Activated Protein Kinase Kinase 4 antibodyAnti-Sapk Kinase 4 antibodyAnti-Sapkk-4 antibodyAnti-Sapkk4 antibodyAnti-C-Jun N-Terminal Kinase Kinase 2 antibodyAnti-Jnk Kinase 2 antibodyAnti-Jnkk 2 antibodyAnti-MAP2K7 antibodyAnti-JNKK2 antibodyAnti-MEK7 antibodyAnti-MKK7 antibodyAnti-PRKMK7 antibodyAnti-SKK4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
