• Western blot analysis of extracts of NIH/3T3 cells, using Phospho-HSL-S853 rabbit monoclonal antibody (STJ11102644) at 1:1000 dilution. NIH/3T3 cells were treated by Forskolin (30 uM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of extracts of NIH/3T3 cells, using Phospho-HSL-S853 Rabbit monoclonal antibody (STJ11102644) at 1:1000 dilution. NIH/3T3 cells were treated by Forskolin (30 uM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-Phospho-LIPE-S853 antibody [S4MR] (STJ11102644)

SKU:
STJ11102644

£146.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Phospho-HSL-S853 is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LIPE
Gene ID: 3991
Uniprot ID: LIPS_HUMAN
Immunogen: A synthetic phosphorylated peptide around S853 of human HSL (Q05469).
Immunogen Sequence: RRSVS
Tissue Specificity Testis.
Post Translational Modifications Phosphorylation by AMPK reduces its translocation towards the lipid droplets.
Function Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters. Shows a preferential hydrolysis of DAGs over TAGs and MAGs and preferentially hydrolyzes the fatty acid (FA) esters at the sn-3 position of the glycerol backbone in DAGs. Preferentially hydrolyzes FA esters at the sn-1 and sn-2 positions of the glycerol backbone in TAGs. Catalyzes the hydrolysis of 2-arachidonoylglycerol, an endocannabinoid and of 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
Protein Name Hormone-Sensitive Lipase
Hsl
Monoacylglycerol Lipase Lipe
Retinyl Ester Hydrolase
Reh
Database Links Reactome: R-HSA-163560
Cellular Localisation Cell Membrane
Membrane
Caveola
Cytoplasm
Cytosol
Lipid Droplet
Found In The High-Density Caveolae
Translocates To The Cytoplasm From The Caveolae Upon Insulin Stimulation
Phosphorylation By Ampk Reduces Its Translocation Towards The Lipid Droplets
Alternative Antibody Names Anti-Hormone-Sensitive Lipase antibody
Anti-Hsl antibody
Anti-Monoacylglycerol Lipase Lipe antibody
Anti-Retinyl Ester Hydrolase antibody
Anti-Reh antibody
Anti-LIPE antibody

Information sourced from Uniprot.org

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