Anti-Phospho-LEF1-Ser42 antibody (8-57 aa) (STJ90534)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human LEF-1 around the phosphorylation site of Ser42 at the amino acid range 8-57
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Lymphoid enhancer-binding factor 1-Ser42 (8-57 aa) for use in WB and IHC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC-P 1:50-300 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | LEF1 |
| Gene ID | 51176 |
| Uniprot ID | LEF1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human LEF-1 around the phosphorylation site of Ser42 at the amino acid range 8-57 |
| Immunogen Region | 8-57 aa |
| Specificity | Phospho-LEF-1 (S42) Polyclonal Antibody detects endogenous levels of LEF-1 protein only when phosphorylated at S42. |
Additional Info
| Post Translational Modifications | Phosphorylated at Thr-155 and/or Ser-166 by NLK. Phosphorylation by NLK at these sites represses LEF1-mediated transcriptional activation of target genes of the canonical Wnt signaling pathway. |
| Function | Transcription factor that binds DNA in a sequence-specific manner. Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Required for IL17A expressing gamma-delta T-cell maturation and development, via binding to regulator loci of BLK to modulate expression. Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, expression is repressed during the bell stage by MSX1-mediated inhibition of CTNNB1 signaling. May play a role in hair cell differentiation and follicle morphogenesis. Isoform 1: Transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells. Isoform 3: Lacks the CTNNB1 interaction domain and may therefore be an antagonist for Wnt signaling. Isoform 5: Transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. |
| Protein Name | Lymphoid Enhancer-Binding Factor 1Lef-1T Cell-Specific Transcription Factor 1-AlphaTcf1-Alpha |
| Database Links | Reactome: R-HSA-201722Reactome: R-HSA-3769402Reactome: R-HSA-4086398Reactome: R-HSA-4411364Reactome: R-HSA-4641265Reactome: R-HSA-8853884Reactome: R-HSA-8951430Reactome: R-HSA-9616222Reactome: R-HSA-9733709Reactome: R-HSA-9754189Reactome: R-HSA-9793380Reactome: R-HSA-9796292Reactome: R-HSA-9824272Reactome: R-HSA-9824585Reactome: R-HSA-9825892Reactome: R-HSA-9856649 |
| Cellular Localisation | NucleusFound In Nuclear Bodies Upon Piasg Binding |
| Alternative Antibody Names | Anti-Lymphoid Enhancer-Binding Factor 1 antibodyAnti-Lef-1 antibodyAnti-T Cell-Specific Transcription Factor 1-Alpha antibodyAnti-Tcf1-Alpha antibodyAnti-LEF1 antibody |
Information sourced from Uniprot.org