| Host: |
Rabbit |
| Applications: |
IHC/IF/ELISA |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Serine/threonine-protein kinase LATS1 and Serine/threonine-protein kinase LATS2-Thr1079/1041 (1041-1090 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
IHC 1:100-1:300ELISA 1:20000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
LATS1LATS2 |
| Gene ID: |
911326524 |
| Uniprot ID: |
LATS1_HUMANLATS2_HUMAN |
| Immunogen Region: |
1041-1090 aa |
| Specificity: |
Phospho-LATS1/2 (T1079/1041) Polyclonal Antibody detects endogenous levels of LATS1/2 protein only when phosphorylated at T1079/1041. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human LATS1/2 around the phosphorylation site of Thr1079/1041 at the amino acid range 1041-1090 |
| Post Translational Modifications | Autophosphorylated and phosphorylated during M-phase of the cell cycle. Phosphorylated by STK3/MST2 at Ser-909 and Thr-1079, which results in its activation. Phosphorylation at Ser-464 by NUAK1 and NUAK2 leads to decreased protein level and is required to regulate cellular senescence and cellular ploidy. |
| Function | Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function. Plays a role in mammary gland epithelial cell differentiation, both through the Hippo signaling pathway and the intracellular estrogen receptor signaling pathway by promoting the degradation of ESR1. |
| Protein Name | Serine/Threonine-Protein Kinase Lats1Large Tumor Suppressor Homolog 1Warts Protein KinaseH-Warts |
| Database Links | Reactome: R-HSA-2028269 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeSpindleMidbodySpindle Pole BodyLocalizes To The Centrosomes Throughout Interphase But Migrates To The Mitotic ApparatusIncluding Spindle Pole BodiesMitotic SpindleAnd MidbodyDuring Mitosis |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Lats1 antibodyAnti-Large Tumor Suppressor Homolog 1 antibodyAnti-Warts Protein Kinase antibodyAnti-H-Warts antibodyAnti-LATS1 antibodyAnti-WARTS antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
