Anti-Phospho-LAT-Tyr200 antibody [R07-3C5] (STJA0034521)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic phosphopeptide corresponding to residues surrounding Tyr200 of human LAT
General Information
| Short Description | Rabbit monoclonal anti-Phospho-LAT-Tyr200 for use in WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R07-3C5 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | LAT |
| Gene ID | 27040 |
| Uniprot ID | LAT_HUMAN |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Tyr200 of human LAT |
Additional Info
| Tissue Specificity | Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level). |
| Post Translational Modifications | Phosphorylated on tyrosines by ZAP70 upon TCR activation, or by SYK upon other immunoreceptor activation.which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ. Phosphorylated by ITK leading to the recruitment of VAV1 to LAT-containing complexes. Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation. 'Lys-63'-linked ubiquitinated by TRAF6. |
| Function | Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules. |
| Protein Name | Linker For Activation Of T-Cells Family Member 136 Kda Phosphotyrosine Adapter ProteinPp36P36-38 |
| Database Links | Reactome: R-HSA-114604Reactome: R-HSA-202433Reactome: R-HSA-2424491 O43561-2Reactome: R-HSA-2454202 O43561-2Reactome: R-HSA-2871796 O43561-2Reactome: R-HSA-2871809 O43561-2Reactome: R-HSA-5673001 O43561-2 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Iii Membrane ProteinPresent In Lipid Rafts |
| Alternative Antibody Names | Anti-Linker For Activation Of T-Cells Family Member 1 antibodyAnti-36 Kda Phosphotyrosine Adapter Protein antibodyAnti-Pp36 antibodyAnti-P36-38 antibodyAnti-LAT antibody |
Information sourced from Uniprot.org