• Immunohistochemistry analysis of paraffin-embedded human brain, using KSR (Phospho-Ser392) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from HepG2 cells, using KSR (Phospho-Ser392) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-Ksr-1 (S392) Polyclonal Antibody

Anti-Phospho-KSR1-Ser392 antibody (358-407 aa) (STJ90763)

SKU:
STJ90763

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Kinase suppressor of Ras 1-Ser392 (358-407 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KSR1
Gene ID: 8844
Uniprot ID: KSR1_HUMAN
Immunogen Region: 358-407 aa
Specificity: Phospho-Ksr-1 (S392) Polyclonal Antibody detects endogenous levels of Ksr-1 protein only when phosphorylated at S392.
Immunogen: The antiserum was produced against synthesized peptide derived from the human KSR around the phosphorylation site of Ser392 at the amino acid range 358-407
Function Part of a multiprotein signaling complex which promotes phosphorylation of Raf family members and activation of downstream MAP kinases. Independently of its kinase activity, acts as MAP2K1/MEK1 and MAP2K2/MEK2-dependent allosteric activator of BRAF.upon binding to MAP2K1/MEK1 or MAP2K2/MEK2, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 and/or MAP2K2/MEK2. Promotes activation of MAPK1 and/or MAPK3, both in response to EGF and to cAMP. Its kinase activity is unsure. Some protein kinase activity has been detected in vitro, however the physiological relevance of this activity is unknown.
Protein Name Kinase Suppressor Of Ras 1
Database Links Reactome: R-HSA-5673000
Reactome: R-HSA-5674135
Reactome: R-HSA-5675221
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-9649948
Reactome: R-HSA-9656223
Cellular Localisation Cytoplasm
Membrane
Peripheral Membrane Protein
Cell Membrane
Cell Projection
Ruffle Membrane
Endoplasmic Reticulum Membrane
In Unstimulated Cells
Where The Phosphorylated Form Is Bound To A 14-3-3 Protein
Sequestration In The Cytoplasm Occurs
Following Growth Factor Treatment
The Protein Is Free For Membrane Translocation
And It Moves From The Cytoplasm To The Cell Periphery
Alternative Antibody Names Anti-Kinase Suppressor Of Ras 1 antibody
Anti-KSR1 antibody
Anti-KSR antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance