• Western blot analysis of lysates from HepG2 cells, using Keratin 18 (Phospho-Ser52) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunofluorescence analysis of HeLa cells, using Keratin 18 (Phospho-Ser52) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of 3T3 293T using p-Cytokeratin 18 (S52) antibody. Antibody was diluted at 1:500

Anti-Phospho-KRT18-Ser52 antibody (21-70 aa) (STJ90707)

SKU:
STJ90707

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Keratin, type I cytoskeletal 18-Ser52 (21-70 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT18
Gene ID: 3875
Uniprot ID: K1C18_HUMAN
Immunogen Region: 21-70 aa
Specificity: Phospho-Cytokeratin 18 (S52) Polyclonal Antibody detects endogenous levels of Cytokeratin 18 protein only when phosphorylated at S52.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Keratin 18 around the phosphorylation site of Ser52 at the amino acid range 21-70
Post Translational Modifications Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7. O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.
Function Involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Protein Name Keratin - Type I Cytoskeletal 18
Cell Proliferation-Inducing Gene 46 Protein
Cytokeratin-18
Ck-18
Keratin-18
K18
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Cellular Localisation Nucleus Matrix
Cytoplasm
Perinuclear Region
Nucleus
Nucleolus
Alternative Antibody Names Anti-Keratin - Type I Cytoskeletal 18 antibody
Anti-Cell Proliferation-Inducing Gene 46 Protein antibody
Anti-Cytokeratin-18 antibody
Anti-Ck-18 antibody
Anti-Keratin-18 antibody
Anti-K18 antibody
Anti-KRT18 antibody
Anti-CYK18 antibody
Anti-PIG46 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance