• Western blot analysis of extracts from HT29 cells untreated or treated with EGF using Phospho-Cytokeratin 18 (KRT18) -S33 antibody (STJ11105775). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA.

Anti-Phospho-KRT18-S33 antibody (STJ11105775)

SKU:
STJ11105775

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Keratin-Type I Cytoskeletal 18-S33 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT18
Gene ID: 3875
Uniprot ID: K1C18_HUMAN
Immunogen: A phospho specific peptide corresponding to residues surrounding S33 of human KRT18
Tissue Specificity Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
Post Translational Modifications Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7. O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.
Function Involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Protein Name Keratin - Type I Cytoskeletal 18
Cell Proliferation-Inducing Gene 46 Protein
Cytokeratin-18
Ck-18
Keratin-18
K18
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Cellular Localisation Nucleus Matrix
Cytoplasm
Perinuclear Region
Nucleus
Nucleolus
Alternative Antibody Names Anti-Keratin - Type I Cytoskeletal 18 antibody
Anti-Cell Proliferation-Inducing Gene 46 Protein antibody
Anti-Cytokeratin-18 antibody
Anti-Ck-18 antibody
Anti-Keratin-18 antibody
Anti-K18 antibody
Anti-KRT18 antibody
Anti-CYK18 antibody
Anti-PIG46 antibody

Information sourced from Uniprot.org

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