• Western blot analysis of lysates from K562 cells treated with forskolin 40nM 30' and 293 cells treated with PMA 125ng/ml 30', using Calsenilin/KCNIP3 (Phospho-Ser63) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human brain, using Calsenilin/KCNIP3 (Phospho-Ser63) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunofluorescence analysis of HeLa cells, using Calsenilin/KCNIP3 (Phospho-Ser63) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of SH-SY5Y 293T 3T3 lysis using Phospho-DREAM (S63) antibody. Antibody was diluted at 1:500

Anti-Phospho-KCNIP3-Ser63 antibody (29-78 aa) (STJ91090)

SKU:
STJ91090

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Calsenilin-Ser63 (29-78 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KCNIP3
Gene ID: 30818
Uniprot ID: CSEN_HUMAN
Immunogen Region: 29-78 aa
Specificity: Phospho-DREAM (S63) Polyclonal Antibody detects endogenous levels of DREAM protein only when phosphorylated at S63.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Calsenilin/KCNIP3 around the phosphorylation site of Ser63 at the amino acid range 29-78
Post Translational Modifications Palmitoylated. Palmitoylation enhances association with the plasma membrane. Proteolytically cleaved by caspase-3. Phosphorylation at Ser-63 inhibits cleavage by CASP3.
Function Calcium-dependent transcriptional repressor that binds to the DRE element of genes including PDYN and FOS. Affinity for DNA is reduced upon binding to calcium and enhanced by binding to magnesium. Seems to be involved in nociception. Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels, such as KCND2/Kv4.2 and KCND3/Kv4.3. Modulates channel expression at the cell membrane, gating characteristics, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. May play a role in the regulation of PSEN2 proteolytic processing and apoptosis. Together with PSEN2 involved in modulation of amyloid-beta formation.
Protein Name Calsenilin
A-Type Potassium Channel Modulatory Protein 3
Dre-Antagonist Modulator
Dream
Kv Channel-Interacting Protein 3
Kchip3
Database Links Reactome: R-HSA-5576894
Reactome: R-HSA-9768777
Cellular Localisation Cytoplasm
Cell Membrane
Lipid-Anchor
Endoplasmic Reticulum
Golgi Apparatus
Nucleus
Also Membrane-Bound
Associated With The Plasma Membrane
In The Presence Of Psen2 Associated With The Endoplasmic Reticulum And Golgi
The Sumoylated Form Is Present Only In The Nucleus
Alternative Antibody Names Anti-Calsenilin antibody
Anti-A-Type Potassium Channel Modulatory Protein 3 antibody
Anti-Dre-Antagonist Modulator antibody
Anti-Dream antibody
Anti-Kv Channel-Interacting Protein 3 antibody
Anti-Kchip3 antibody
Anti-KCNIP3 antibody
Anti-CSEN antibody
Anti-DREAM antibody
Anti-KCHIP3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance