• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using JunB (Phospho-Ser259) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from HeLa cells, using JunB (Phospho-Ser259) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-Jun B (S259) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-JUNB-Ser259 antibody (226-275 aa) (STJ90318)

SKU:
STJ90318

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Transcription factor JunB-Ser259 (226-275 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IP 2-5 ug mg/lysate
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: JUNB
Gene ID: 3726
Uniprot ID: JUNB_HUMAN
Immunogen Region: 226-275 aa
Specificity: Phospho-Jun B (S259) Polyclonal Antibody detects endogenous levels of Jun B protein only when phosphorylated at S259.
Immunogen: The antiserum was produced against synthesized peptide derived from the human JunB around the phosphorylation site of Ser259 at the amino acid range 226-275
Function Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGAGCTCA-3'. Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to an AP-1 consensus sequence and its transcriptional activity.
Protein Name Transcription Factor Junb
Transcription Factor Ap-1 Subunit Junb
Database Links Reactome: R-HSA-2173796
Reactome: R-HSA-6785807
Reactome: R-HSA-9031628
Reactome: R-HSA-9725371
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor Junb antibody
Anti-Transcription Factor Ap-1 Subunit Junb antibody
Anti-JUNB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance