| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-IRF7-S471/472 is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
IRF7 |
| Gene ID: |
3665 |
| Uniprot ID: |
IRF7_HUMAN |
| Immunogen: |
A synthetic phosphorylated peptide around S471 & S472 of human IRF7 (NP_001563.2). |
| Immunogen Sequence: |
GVSSLD |
| Tissue Specificity | Expressed predominantly in spleen, thymus and peripheral blood leukocytes. |
| Post Translational Modifications | Acetylation inhibits its DNA-binding ability and activity. In response to a viral infection, phosphorylated on Ser-477 and Ser-479 by TBK1 and IKBKE1. Phosphorylation, and subsequent activation is inhibited by vaccinia virus protein E3. In TLR7- and TLR9-mediated signaling pathway, phosphorylated by IRAK1. TRAF6-mediated ubiquitination is required for IRF7 activation. TRIM35 mediates IRF7 'Lys-48'-linked polyubiquitination and subsequent proteasomal degradation. Ubiquitinated by UBE3C, leading to its degradation. Sumoylated by TRIM28, which inhibits its transactivation activity. (Microbial infection) Cleaved and inactivated by the protease 3C of enterovirus 71 allowing the virus to disrupt the host type I interferon production. (Microbial infection) Cleaved and inactivated by the protease 3C of human enterovirus 68D (EV68) allowing the virus to disrupt the host type I interferon production. 'Lys-48'-linked polyubiquitination and subsequent proteasomal degradation is NMI-dependent in response to Sendai virus infection. 'Lys-63'-linked ubiquitination by NEURL3 promotes IRF7 activation. |
| Function | Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction via both the virus-activated, MyD88-independent pathway and the TLR-activated, MyD88-dependent pathway. Induces transcription of ubiquitin hydrolase USP25 mRNA in response to lipopolysaccharide (LPS) or viral infection in a type I IFN-dependent manner. Required during both the early and late phases of the IFN gene induction but is more critical for the late than for the early phase. Exists in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization where along with other coactivators it can activate transcription of the type I IFN and ISG genes. Can also play a role in regulating adaptive immune responses by inducing PSMB9/LMP2 expression, either directly or through induction of IRF1. Binds to the Q promoter (Qp) of EBV nuclear antigen 1 a (EBNA1) and may play a role in the regulation of EBV latency. Can activate distinct gene expression programs in macrophages and regulate the anti-tumor properties of primary macrophages. |
| Protein Name | Interferon Regulatory Factor 7Irf-7 |
| Database Links | Reactome: R-HSA-3134963Reactome: R-HSA-877300Reactome: R-HSA-9013973Reactome: R-HSA-909733Reactome: R-HSA-918233Reactome: R-HSA-933541Reactome: R-HSA-936964Reactome: R-HSA-9705671Reactome: R-HSA-975110 |
| Cellular Localisation | NucleusCytoplasmThe Phosphorylated And Active Form Accumulates Selectively In The Nucleus |
| Alternative Antibody Names | Anti-Interferon Regulatory Factor 7 antibodyAnti-Irf-7 antibodyAnti-IRF7 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
