• Western blot analysis of extracts of various cell lines, using Phospho-IRF7-S471/472 antibody (STJ11103778) at 1:500 dilution. 293T and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of various lysates using Phospho-IRF7-S471/472 Rabbit polyclonal antibody (STJ11103778) at 1:500 dilution. 293T and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-IRF7-S471/472 antibody (STJ11103778)

SKU:
STJ11103778

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-IRF7-S471/472 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IRF7
Gene ID: 3665
Uniprot ID: IRF7_HUMAN
Immunogen: A synthetic phosphorylated peptide around S471 & S472 of human IRF7 (NP_001563.2).
Immunogen Sequence: GVSSLD
Tissue Specificity Expressed predominantly in spleen, thymus and peripheral blood leukocytes.
Post Translational Modifications Acetylation inhibits its DNA-binding ability and activity. In response to a viral infection, phosphorylated on Ser-477 and Ser-479 by TBK1 and IKBKE1. Phosphorylation, and subsequent activation is inhibited by vaccinia virus protein E3. In TLR7- and TLR9-mediated signaling pathway, phosphorylated by IRAK1. TRAF6-mediated ubiquitination is required for IRF7 activation. TRIM35 mediates IRF7 'Lys-48'-linked polyubiquitination and subsequent proteasomal degradation. Ubiquitinated by UBE3C, leading to its degradation. Sumoylated by TRIM28, which inhibits its transactivation activity. (Microbial infection) Cleaved and inactivated by the protease 3C of enterovirus 71 allowing the virus to disrupt the host type I interferon production. (Microbial infection) Cleaved and inactivated by the protease 3C of human enterovirus 68D (EV68) allowing the virus to disrupt the host type I interferon production. 'Lys-48'-linked polyubiquitination and subsequent proteasomal degradation is NMI-dependent in response to Sendai virus infection. 'Lys-63'-linked ubiquitination by NEURL3 promotes IRF7 activation.
Function Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction via both the virus-activated, MyD88-independent pathway and the TLR-activated, MyD88-dependent pathway. Induces transcription of ubiquitin hydrolase USP25 mRNA in response to lipopolysaccharide (LPS) or viral infection in a type I IFN-dependent manner. Required during both the early and late phases of the IFN gene induction but is more critical for the late than for the early phase. Exists in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization where along with other coactivators it can activate transcription of the type I IFN and ISG genes. Can also play a role in regulating adaptive immune responses by inducing PSMB9/LMP2 expression, either directly or through induction of IRF1. Binds to the Q promoter (Qp) of EBV nuclear antigen 1 a (EBNA1) and may play a role in the regulation of EBV latency. Can activate distinct gene expression programs in macrophages and regulate the anti-tumor properties of primary macrophages.
Protein Name Interferon Regulatory Factor 7
Irf-7
Database Links Reactome: R-HSA-3134963
Reactome: R-HSA-877300
Reactome: R-HSA-9013973
Reactome: R-HSA-909733
Reactome: R-HSA-918233
Reactome: R-HSA-933541
Reactome: R-HSA-936964
Reactome: R-HSA-9705671
Reactome: R-HSA-975110
Cellular Localisation Nucleus
Cytoplasm
The Phosphorylated And Active Form Accumulates Selectively In The Nucleus
Alternative Antibody Names Anti-Interferon Regulatory Factor 7 antibody
Anti-Irf-7 antibody
Anti-IRF7 antibody

Information sourced from Uniprot.org

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