• Immunohistochemistry analysis of paraffin-embedded human brain, using IKK-gamma (Phospho-Ser376) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-IKBKG-Ser376 antibody (342-391 aa) (STJ91203)

SKU:
STJ91203

Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-NF-kappa-B essential modulator-Ser376 (342-391 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IKBKG
Gene ID: 8517
Uniprot ID: NEMO_HUMAN
Immunogen Region: 342-391 aa
Specificity: Phospho-IKK Gamma (S376) Polyclonal Antibody detects endogenous levels of IKK Gamma protein only when phosphorylated at S376.
Immunogen: The antiserum was produced against synthesized peptide derived from the human IKK-gamma around the phosphorylation site of Ser376 at the amino acid range 342-391
Post Translational Modifications Phosphorylation at Ser-68 attenuates aminoterminal homodimerization. Polyubiquitinated on Lys-285 via 'Lys-63'-linked ubiquitin.the ubiquitination is mediated downstream of NOD2 and RIPK2 and probably plays a role in signaling by facilitating interactions with ubiquitin domain-containing proteins and activates the NF-kappa-B pathway. Polyubiquitinated on Lys-285 and Lys-399 through 'Lys-63'-linked ubiquitin.the ubiquitination is mediated by BCL10, MALT1 and TRAF6 and probably plays a role in signaling by facilitating interactions with ubiquitin domain-containing proteins and activates the NF-kappa-B pathway. Monoubiquitinated on Lys-277 and Lys-309.promotes nuclear export. Polyubiquitinated through 'Lys-27' by TRIM23.involved in antiviral innate and inflammatory responses. Linear polyubiquitinated on Lys-111, Lys-143, Lys-226, Lys-246, Lys-264, Lys-277, Lys-285, Lys-292, Lys-302, Lys-309 and Lys-326.the head-to-tail polyubiquitination is mediated by the LUBAC complex and plays a key role in NF-kappa-B activation. Deubiquitinated by USP10 in a TANK-dependent and -independent manner, leading to the negative regulation of NF-kappa-B signaling upon DNA damage. Ubiquitinated at Lys-326 by MARCHF2 following bacterial and viral infection which leads to its degradation. Polyubiquitinated via 'Lys-29'-linked ubiquitin.leading to lysosomal degradation. Sumoylated on Lys-277 and Lys-309 with SUMO1.the modification results in phosphorylation of Ser-85 by ATM leading to a replacement of the sumoylation by mono-ubiquitination on these residues. Neddylated by TRIM40, resulting in stabilization of NFKBIA and down-regulation of NF-kappa-B activity. (Microbial infection) Cleaved by hepatitis A virus (HAV) protease 3C allowing the virus to disrupt the host innate immune signaling. (Microbial infection) Deubiquitinated by Epstein-Barr virus BPLF1 on both 'Lys-48' and 'Lys-63'-linked ubiquitin chains.leading to NF-kappa-B signaling inhibition. (Microbial infection) Polyubiquitinated on Lys-309 and Lys-321 via 'Lys-27'-linked ubiquitin by Shigella flexneri E3 ubiquitin-protein ligase ipah9.8, leading to its degradation by the proteasome. (Microbial infection) Polyubiquitination through 'Lys-63' is interrupted by interaction with SARS coronavirus-2/SARS-CoV-2 virus protein ORF9B which inhibits the NF-kappa-B pathway.
Function Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin plays a key role in IKK activation by multiple signaling receptor pathways. Can recognize and bind both 'Lys-63'-linked and linear polyubiquitin upon cell stimulation, with a much higher affinity for linear polyubiquitin. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response.this function requires 'Lys-27'-linked polyubiquitination. (Microbial infection) Also considered to be a mediator for HTLV-1 Tax oncoprotein activation of NF-kappa-B.
Protein Name Nf-Kappa-B Essential Modulator
Nemo
Fip-3
Ikb Kinase-Associated Protein 1
Ikkap1
Inhibitor Of Nuclear Factor Kappa-B Kinase Subunit Gamma
I-Kappa-B Kinase Subunit Gamma
Ikk-Gamma
Ikkg
Ikb Kinase Subunit Gamma
Nf-Kappa-B Essential Modifier
Database Links Reactome: R-HSA-1169091
Reactome: R-HSA-1236974
Reactome: R-HSA-168638
Reactome: R-HSA-168927
Reactome: R-HSA-1810476
Reactome: R-HSA-202424
Reactome: R-HSA-2871837
Reactome: R-HSA-445989
Reactome: R-HSA-450302
Reactome: R-HSA-450321
Reactome: R-HSA-4755510
Reactome: R-HSA-5357905
Reactome: R-HSA-5357956
Reactome: R-HSA-5602636
Reactome: R-HSA-5603027
Reactome: R-HSA-5603029
Reactome: R-HSA-5607764
Reactome: R-HSA-5684264
Reactome: R-HSA-5689880
Reactome: R-HSA-5689896
Reactome: R-HSA-9020702
Reactome: R-HSA-933542
Reactome: R-HSA-933543
Reactome: R-HSA-937039
Reactome: R-HSA-937041
Reactome: R-HSA-9705671
Reactome: R-HSA-975144
Reactome: R-HSA-9758274
Cellular Localisation Cytoplasm
Nucleus
Sumoylated Nemo Accumulates In The Nucleus In Response To Genotoxic Stress
Alternative Antibody Names Anti-Nf-Kappa-B Essential Modulator antibody
Anti-Nemo antibody
Anti-Fip-3 antibody
Anti-Ikb Kinase-Associated Protein 1 antibody
Anti-Ikkap1 antibody
Anti-Inhibitor Of Nuclear Factor Kappa-B Kinase Subunit Gamma antibody
Anti-I-Kappa-B Kinase Subunit Gamma antibody
Anti-Ikk-Gamma antibody
Anti-Ikkg antibody
Anti-Ikb Kinase Subunit Gamma antibody
Anti-Nf-Kappa-B Essential Modifier antibody
Anti-IKBKG antibody
Anti-FIP3 antibody
Anti-NEMO antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance