| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Inhibitor of nuclear factor kappa-B kinase subunit beta-Tyr199 (166-215 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
IKBKB |
| Gene ID: |
3551 |
| Uniprot ID: |
IKKB_HUMAN |
| Immunogen Region: |
166-215 aa |
| Specificity: |
Phospho-IKK Beta (Y199) Polyclonal Antibody detects endogenous levels of IKK Beta protein only when phosphorylated at Y199. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human IKK-beta around the phosphorylation site of Tyr199 at the amino acid range 166-215 |
| Post Translational Modifications | Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK as well as TBK1 and PRKCZ.which enhances activity. Phosphorylated by MAP3K7/TAK1 in response to NOD1 and NOD2 signaling, promoting activation and phosphorylation of NF-kappa-B inhibitors, leading to NF-kappa-B activation. Once activated, autophosphorylates on the C-terminal serine cluster.which decreases activity and prevents prolonged activation of the inflammatory response. Phosphorylated by the IKK-related kinases TBK1 and IKBKE, which is associated with reduced CHUK/IKKA and IKBKB activity and NF-kappa-B-dependent gene transcription. Dephosphorylated at Ser-177 and Ser-181 by PPM1A and PPM1B. (Microbial infection) Acetylation of Thr-180 by Yersinia YopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway. Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. 'Ser-163' does not serve as a monoubiquitination site. ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues. (Microbial infection) Monoubiquitination by TRIM21 is disrupted by Yersinia YopJ. Hydroxylated by PHD1/EGLN2, loss of hydroxylation under hypoxic conditions results in activation of NF-kappa-B. |
| Function | Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation. Phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor. Also phosphorylates other substrates including NAA10, NCOA3, BCL10 and IRS1. Phosphorylates RIPK1 at 'Ser-25' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death. Phosphorylates the C-terminus of IRF5, stimulating IRF5 homodimerization and translocation into the nucleus. |
| Protein Name | Inhibitor Of Nuclear Factor Kappa-B Kinase Subunit BetaI-Kappa-B-Kinase BetaIkk-BIkk-BetaIkbkbI-Kappa-B Kinase 2Ikk-2Ikk2Nuclear Factor Nf-Kappa-B Inhibitor Kinase BetaNfkbikbSerine/Threonine Protein Kinase Ikbkb |
| Database Links | Reactome: R-HSA-1169091Reactome: R-HSA-1236974Reactome: R-HSA-168638Reactome: R-HSA-168927Reactome: R-HSA-1810476Reactome: R-HSA-202424Reactome: R-HSA-209543Reactome: R-HSA-209560Reactome: R-HSA-2871837Reactome: R-HSA-445989Reactome: R-HSA-5357905Reactome: R-HSA-5357956Reactome: R-HSA-5602636Reactome: R-HSA-5603027Reactome: R-HSA-5603029Reactome: R-HSA-5607764Reactome: R-HSA-5684264Reactome: R-HSA-9020702Reactome: R-HSA-933542Reactome: R-HSA-933543Reactome: R-HSA-937039Reactome: R-HSA-937041Reactome: R-HSA-9705671Reactome: R-HSA-975144Reactome: R-HSA-9758274 |
| Cellular Localisation | CytoplasmNucleusMembrane RaftColocalized With Dpp4 In Membrane Rafts |
| Alternative Antibody Names | Anti-Inhibitor Of Nuclear Factor Kappa-B Kinase Subunit Beta antibodyAnti-I-Kappa-B-Kinase Beta antibodyAnti-Ikk-B antibodyAnti-Ikk-Beta antibodyAnti-Ikbkb antibodyAnti-I-Kappa-B Kinase 2 antibodyAnti-Ikk-2 antibodyAnti-Ikk2 antibodyAnti-Nuclear Factor Nf-Kappa-B Inhibitor Kinase Beta antibodyAnti-Nfkbikb antibodyAnti-Serine/Threonine Protein Kinase Ikbkb antibodyAnti-IKBKB antibodyAnti-IKKB antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
