| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human/Mouse/Rat/Monkey |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Inhibitor of nuclear factor kappa-B kinase subunit beta-Tyr188 (161-210 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
IKBKB |
| Gene ID: |
3551 |
| Uniprot ID: |
IKKB_HUMAN |
| Immunogen Region: |
161-210 aa |
| Specificity: |
Phospho-IKK Beta (Y188) Polyclonal Antibody detects endogenous levels of IKK Beta protein only when phosphorylated at Y188. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human IKK-beta around the phosphorylation site of Tyr188 at the amino acid range 161-210 |
| Post Translational Modifications | Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK as well as TBK1 and PRKCZ.which enhances activity. Phosphorylated by MAP3K7/TAK1 in response to NOD1 and NOD2 signaling, promoting activation and phosphorylation of NF-kappa-B inhibitors, leading to NF-kappa-B activation. Once activated, autophosphorylates on the C-terminal serine cluster.which decreases activity and prevents prolonged activation of the inflammatory response. Phosphorylated by the IKK-related kinases TBK1 and IKBKE, which is associated with reduced CHUK/IKKA and IKBKB activity and NF-kappa-B-dependent gene transcription. Dephosphorylated at Ser-177 and Ser-181 by PPM1A and PPM1B. (Microbial infection) Acetylation of Thr-180 by Yersinia YopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway. Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. 'Ser-163' does not serve as a monoubiquitination site. ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues. (Microbial infection) Monoubiquitination by TRIM21 is disrupted by Yersinia YopJ. Hydroxylated by PHD1/EGLN2, loss of hydroxylation under hypoxic conditions results in activation of NF-kappa-B. |
| Function | Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as a part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation. Phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor. Also phosphorylates other substrates including NAA10, NCOA3, BCL10 and IRS1. Phosphorylates RIPK1 at 'Ser-25' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death. Phosphorylates the C-terminus of IRF5, stimulating IRF5 homodimerization and translocation into the nucleus. Following bacterial lipopolysaccharide (LPS)-induced TLR4 endocytosis, phosphorylates STAT1 at 'Thr-749' which restricts interferon signaling and anti-inflammatory responses and promotes innate inflammatory responses. IKBKB-mediated phosphorylation of STAT1 at 'Thr-749' promotes binding of STAT1 to the ARID5A promoter, resulting in transcriptional activation of ARID5A and subsequent ARID5A-mediated stabilization of IL6. It also promotes binding of STAT1 to the IL12B promoter and activation of IL12B transcription. |
| Protein Name | Inhibitor Of Nuclear Factor Kappa-B Kinase Subunit BetaI-Kappa-B-Kinase BetaIkk-BIkk-BetaIkbkbI-Kappa-B Kinase 2Ikk-2Ikk2Nuclear Factor Nf-Kappa-B Inhibitor Kinase BetaNfkbikbSerine/Threonine Protein Kinase Ikbkb |
| Database Links | Reactome: R-HSA-1169091Reactome: R-HSA-1236974Reactome: R-HSA-168638Reactome: R-HSA-168927Reactome: R-HSA-1810476Reactome: R-HSA-202424Reactome: R-HSA-209543Reactome: R-HSA-209560Reactome: R-HSA-2871837Reactome: R-HSA-445989Reactome: R-HSA-5357905Reactome: R-HSA-5357956Reactome: R-HSA-5602636Reactome: R-HSA-5603027Reactome: R-HSA-5603029Reactome: R-HSA-5607764Reactome: R-HSA-5684264Reactome: R-HSA-9020702Reactome: R-HSA-933542Reactome: R-HSA-933543Reactome: R-HSA-937039Reactome: R-HSA-937041Reactome: R-HSA-9705671Reactome: R-HSA-975144Reactome: R-HSA-9758274Reactome: R-HSA-9833482Reactome: R-HSA-9860276 |
| Cellular Localisation | CytoplasmNucleusMembrane RaftColocalized With Dpp4 In Membrane Rafts |
| Alternative Antibody Names | Anti-Inhibitor Of Nuclear Factor Kappa-B Kinase Subunit Beta antibodyAnti-I-Kappa-B-Kinase Beta antibodyAnti-Ikk-B antibodyAnti-Ikk-Beta antibodyAnti-Ikbkb antibodyAnti-I-Kappa-B Kinase 2 antibodyAnti-Ikk-2 antibodyAnti-Ikk2 antibodyAnti-Nuclear Factor Nf-Kappa-B Inhibitor Kinase Beta antibodyAnti-Nfkbikb antibodyAnti-Serine/Threonine Protein Kinase Ikbkb antibodyAnti-IKBKB antibodyAnti-IKKB antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
