• Western blot analysis of extracts of 293T cells, using Phospho-INSR-Y1189/Y1190 + IGF1R-Y1165/Y1166 antibody (STJ11101123) at 1:1000 dilution or IGF1R antibody (A0243). 293T cells were treated by IGF-1 (50 ng/mL) at 37 °C for 5 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of lysates from 293T cells, using Phospho-INSR-Y1189/Y1190 + IGF1R-Y1165/Y1166 Rabbit polyclonal antibody (STJ11101123) at 1:1000 dilution or IGF1R antibody (A0243). 293T cells were treated by IGF-1 (50 ng/mL) at 37 °C for 5 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-IGF1R/INSR-Y1189/Y1190 & Y1165/Y1166 antibody (STJ11101123)

SKU:
STJ11101123

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: INSR
IGF1R
Gene ID: 3643
3480
Uniprot ID: INSR_HUMAN
IGF1R_HUMAN
Specificity: A synthetic phosphorylated peptide around Y1165 & Y1166 of human IGF1RINSR (NP_000199.2).
Immunogen Sequence: YETDYY
Tissue Specificity P06213 - Isoform Long and isoform Short are predominantly expressed in tissue targets of insulin metabolic effects: liver, adipose tissue and skeletal muscle but are also expressed in the peripheral nerve, kidney, pulmonary alveoli, pancreatic acini, placenta vascular endothelium, fibroblasts, monocytes, granulocytes, erythrocytes and skin. Isoform Short is preferentially expressed in fetal cells such as fetal fibroblasts, muscle, liver and kidney. Found as a hybrid receptor with IGF1R in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Overexpressed in several tumors, including breast, colon, lung, ovary, and thyroid carcinomas.
P08069 - Found as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Expressed in a variety of tissues. Overexpressed in tumors, including melanomas, cancers of the colon, pancreas prostate and kidney.
Alternative Antibody Names Anti-Insulin Receptor antibody
Anti-Ir antibody
Anti-Cd Antigen Cd220 Cleaved Into - Insulin Receptor Subunit Alpha - Insulin Receptor Subunit Beta antibody
Anti-INSR antibody
Anti-Insulin-Like Growth Factor 1 Receptor antibody
Anti-Insulin-Like Growth Factor I Receptor antibody
Anti-Igf-I Receptor antibody
Anti-Cd Antigen Cd221 Cleaved Into - Insulin-Like Growth Factor 1 Receptor Alpha Chain - Insulin-Like Growth Factor 1 Receptor Beta Chain antibody
Anti-IGF1R antibody

Information sourced from Uniprot.org

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