Anti-Phospho-HTT-Ser421 antibody (387-436 aa) (STJ91160)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human Huntingtin around the phosphorylation site of Ser421 at the amino acid range 387-436
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Huntingtin-Ser421 (387-436 aa) for use in IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HTT |
| Gene ID | 3064 |
| Uniprot ID | HD_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human Huntingtin around the phosphorylation site of Ser421 at the amino acid range 387-436 |
| Immunogen Region | 387-436 aa |
| Specificity | Phospho-Huntingtin (S421) Polyclonal Antibody detects endogenous levels of Huntingtin protein only when phosphorylated at S421. |
Additional Info
| Post Translational Modifications | Huntingtin: Cleaved by caspases downstream of the polyglutamine stretch. The resulting N-terminal fragments are cytotoxic and provokes apoptosis. Huntingtin: Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation. Huntingtin: Phosphorylation at Ser-1179 and Ser-1199 by CDK5 in response to DNA damage in nuclei of neurons protects neurons against polyglutamine expansion as well as DNA damage mediated toxicity. Huntingtin, myristoylated N-terminal fragment: Myristoylated at Gly-551, following proteolytic cleavage at Asp-550. |
| Function | Huntingtin: May play a role in microtubule-mediated transport or vesicle function. Huntingtin, myristoylated N-terminal fragment: Promotes the formation of autophagic vesicles. |
| Protein Name | HuntingtinHuntington Disease ProteinHd Protein Cleaved Into - Huntingtin - Myristoylated N-Terminal Fragment |
| Database Links | Reactome: R-HSA-9022692 |
| Cellular Localisation | Huntingtin: CytoplasmNucleusEarly EndosomeThe Mutant Huntingtin Protein Colocalizes With Akap8l In The Nuclear Matrix Of Huntington Disease NeuronsShuttles Between Cytoplasm And Nucleus In A Ran Gtpase-Independent MannerRecruits Onto Early Endosomes In A Rab5- And Hap40-Dependent FashionHuntingtinMyristoylated N-Terminal Fragment: Cytoplasmic VesicleAutophagosome |
| Alternative Antibody Names | Anti-Huntingtin antibodyAnti-Huntington Disease Protein antibodyAnti-Hd Protein Cleaved Into - Huntingtin - Myristoylated N-Terminal Fragment antibodyAnti-HTT antibodyAnti-HD antibodyAnti-IT15 antibody |
Information sourced from Uniprot.org