• Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-Phospho-HTRA2-Ser212 antibody (181-230 aa) (STJ91257)

SKU:
STJ91257

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Serine protease HTRA2, mitochondrial-Ser212 (181-230 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HTRA2
Gene ID: 27429
Uniprot ID: HTRA2_HUMAN
Immunogen Region: 181-230 aa
Specificity: Phospho-HtrA2 (S212) Polyclonal Antibody detects endogenous levels of HtrA2 protein only when phosphorylated at S212.
Immunogen: The antiserum was produced against synthesized peptide derived from the human HtrA2 around the phosphorylation site of Ser212 at the amino acid range 181-230
Post Translational Modifications Autoproteolytically activated.
Function Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive.
Protein Name Serine Protease Htra2 - Mitochondrial
High Temperature Requirement Protein A2
Htra2
Omi Stress-Regulated Endoprotease
Serine Protease 25
Serine Proteinase Omi
Cellular Localisation Mitochondrion Intermembrane Space
Mitochondrion Membrane
Single-Pass Membrane Protein
Predominantly Present In The Intermembrane Space
Released Into The Cytosol Following Apoptotic Stimuli
Such As Uv Treatment
And Stimulation Of Mitochondria With Caspase-8 Truncated Bid/Tbid
Isoform 1: Endoplasmic Reticulum
Alternative Antibody Names Anti-Serine Protease Htra2 - Mitochondrial antibody
Anti-High Temperature Requirement Protein A2 antibody
Anti-Htra2 antibody
Anti-Omi Stress-Regulated Endoprotease antibody
Anti-Serine Protease 25 antibody
Anti-Serine Proteinase Omi antibody
Anti-HTRA2 antibody
Anti-OMI antibody
Anti-PRSS25 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance